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| 猪前脂肪细胞原代培养方法的建立 | |
| 引用本文： | 杨红文,蒋思文,高其双,刘则学.猪前脂肪细胞原代培养方法的建立[J].贵州畜牧兽医,2011,35(1):9-11. |
| 作者姓名： | 杨红文 蒋思文 高其双 刘则学 |
| 作者单位： | 1. 华中农业大学动物科技学院,湖北,武汉,430070;贵州省畜牧兽医研究所,贵州,贵阳,550005 2. 华中农业大学动物科技学院,湖北,武汉,430070 3. 武汉市畜牧科学研究所,湖北,武汉,430065 |
| 摘 要： | 目的:摸索猪前脂肪细胞的原代培养方法,为研究猪脂肪发生的分子机制奠定基础.方法:取皮下脂肪,酶消化法分离细胞,用转铁蛋白、氢化可的松、胰岛素诱导分化,油红O染色鉴定.结果:在培养期间,细胞逐渐由梭形变为圆形,并逐渐增大,油红O染色为红色.结论:分离的细胞为未分化的前脂肪细胞,可用此方法培养的细胞进行脂肪发育过程中具体分... |
| 关 键 词： | 猪 前脂肪细胞 细胞培养 鉴定 |
Establishment of Primary Cultivation Method of Porcine Preadipocyte |
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| Yang Hongwen,Jiang Siwen,Gao Qishuang,Liu Zexue.Establishment of Primary Cultivation Method of Porcine Preadipocyte[J].Guizhou Animal Science and Veterinary Medicine,2011,35(1):9-11. | |
| Authors: | Yang Hongwen Jiang Siwen Gao Qishuang Liu Zexue |
| Institution: | 1(1.Department of Animal Sciencce,Huazhong Agricultural University,Wuhan,430070,China;2.Institute of Guizhou Animal Science and veterinary,Gaigang,Guizhou,550005,China;3.Institute of Animal Science,Wuhan Academy of Agricultural Science,Wuhan,430065,China) |
| Abstract: | Objective to establish a primary porcine preadipocyte culture method for better understanding the molecular events of adipogenesis.Methods porcine preadipocyte were iso-lated from subcutaneous adipose using enzemy digeslated method,cultured in 10% fetal bovine serum(FBS)-DMEM/F-12 medium supplemented insulin,transferring and hydrocortisone and identified using Oil Red O.Results cultured fibroblast-like cells were became round during induced differentiation period,the rounded cells were red by Oil Red O staining Conclusion the cultured fibroblast-like cells were preadipocyte.,it could be used to further study for adipose. |
| Keywords: | Porcine Preadipocyte Cell culture |
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