Self-incompatibility genotype identification and stability as influenced by inbreeding in red clover (Trifolium pratense L.) |
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Authors: | M K Anderson N L Taylor J F Duncan |
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Institution: | (1) Department of Agronomy, University of Kentucky, Lexington, Kentucky, USA |
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Abstract: | Summary
S allele genotypes of I1 progenies from eight I0 red clover (Trifolium pratense L.) clones were determined under isolated field conditions. Each I1 progeny was vegetatively increased and isolated under a cage for pollination by honey bees. Clones within each I1 progeny producing relatively large and small amounts of seed were classified homozygous and heterozygous, respectively, for S allele genotype. S allele genotypes were verified by extensive sib crosses in the greenhouse, and almost complete agreement was found with the field classification. I2 progenies were reciprocally test-crossed with their parental I0 clones to detect any changes in S specificity and also to confirm the previous S genotype classifications in the I1 generation. It was concluded that the reliability of field and greenhouse sib classification of S genotypes is based on the strength of the incompatibility reaction in each particular clone. Most I1's showed a strong incompatibility reaction as evidenced by low seed set for heterozygous S genotypes, but one progeny showed a weak incompatibility reaction which resulted in S genotype misclassifications. An S specificity was changed in one I2 progeny.Contribution from the Kentucky Agricultural Experiment Station. This paper (72-3-151) is published with the approval of the Director, Kentucky Agricultural Experiment Station, Lexington, Kentucky 40506, USA. |
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