| 鸭疫里氏杆菌PCR快速检测方法的建立 |
| |
| 引用本文： | 王璇,徐景峨,潘淑惠,吴位珩,杨莉.鸭疫里氏杆菌PCR快速检测方法的建立[J].中国畜牧兽医,2011,38(12):100-102. |
| |
| 作者姓名： | 王璇 徐景峨 潘淑惠 吴位珩 杨莉 |
| |
| 作者单位： | 贵州省畜牧兽医研究所,贵州贵阳 550005 |
| |
| 基金项目： | 贵州省科学技术基金项目，贵州省农科院重点项目，贵州省畜禽健康养殖技术创新能力建设项目，中央补助地方科技基础条件专项基金项目 |
| |
| 摘 要： | 本试验根据GenBank中的鸭疫里氏杆菌主要外膜蛋白A基因序列,在其保守区设计了1对引物,建立了检测鸭疫里氏杆菌的PCR方法,对鸭疫里氏杆菌进行检测,结果能扩增出预期片段,而金黄色葡萄球菌、链球菌、多杀性巴氏杆菌、沙门氏菌、大肠杆菌的扩增结果均为阴性。说明该PCR法具有较好的特异性,可用于鸭疫里氏杆菌感染的检测、分子流行病学调查和分离菌株的快速鉴定。
|
| 关 键 词： | 鸭疫里氏杆菌 检测 聚合酶链式反应 |
Establishment of PCR Method for Quick Detection of Riemerella anatipestifer |
| |
| WANG Xuan
,
XU Jing-e
,
PAN Shu-hui
,
WU Wei-heng
,
YANG Li.Establishment of PCR Method for Quick Detection of Riemerella anatipestifer[J].China Animal Husbandry & Veterinary Medicine,2011,38(12):100-102. |
| |
| Authors: | WANG Xuan XU Jing-e PAN Shu-hui WU Wei-heng YANG Li |
| |
| Institution: | Institute of Animal Husbandry and Veterinary Medicine in Guizhou Province,Guiyang 550005, China |
| |
| Abstract: | According to the gene sequences of the outer membrane protein A from Riemerella anatipestifer(RA) in GenBank, one pair of primers were selected and a specific PCR assay was made. Riemerella anatipestifer were found positive in the PCR. Staphyloccocus aureus, Rosenbach, Streptococcus, Pasteurella Multocida, Salmonella and Escherichia coli were all found negative in the PCR. The specificity and reproduction of PCR suggest it can be used for routine identification of Riemerella anatipestifer. |
| |
| Keywords: | Riemerella anatipestifer detection PCR |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
