猪瘟病毒感染后对机体免疫系统的影响

为初步探讨猪瘟病毒的致病机理及机体的免疫应答机制,进行了猪瘟病毒感染试验,利用流式细胞术、阻断ELISA、白细胞计数和剖检观察对猪的体液免疫、细胞免疫、白细胞数量及病理损伤进行了研究。结果表明,感染猪的CD3^＋和CD4^＋T细胞亚群的数量在感染过程中均出现了明显的降低;CD8^＋T细胞亚群在感染初期变化幅度不大,后期明显升高;猪瘟抗体于第7天开始产生,呈上升趋势。白细胞数量在感染期间呈下降趋势。病理学观察可知感染猪的肺脏、肾脏、扁桃体等出现了不同程度的损伤。猪瘟病毒感染对机体的免疫系统造成了较大的损伤,抑制了机体的免疫应答。

The Immunological Characteristics of Swine Infected by Classical Swine Fever Virus

To investigate the mechanism of pathogenesis and immune responses caused by classical swine fever virus (CSFV),pigs were experimentally infected with CSFV strain in this study. Inoculated pigs were studied for their humoral and cell-mediated immunity to the virus,using FACS on cytokine marker strained lymphocytes,blocking ELISA and leukocyte count. Pathogenesis evaluation on various tissues and organs were conducted for the pathogenesis of CSFV. Results showed that percentages of CD3^＋,CD4^＋lymphocytes significantly decreased in the infected group,while there was an increase in the percentage of CD8^＋ lymphocytes in laer period of infection. The level of ELISA antibody of CSFV increased from 7 to 14 days after challenge,but the situation of animals did not get better. Total WBC dropped in the experimentally infected pigs during the experiment. Pathological observations demonstrated various degree lesions,bleeding and necrosis in the most of organs from the infected pigs,such as lung,kidney,tonsil and lymph nodes. Those results indicated that CSFV infection would affect innate and adaptive immune responses and cause pathological lesions in the infected pigs.