鸡脂滴包被蛋白基因内含子5多态性与胴体及脂肪性状的相关性分析

本试验采用PCR-RFLP方法检测脂滴包被蛋白基因(perilipin,PLIN)在济宁百日鸡、汶上芦花鸡等5个地方鸡种和1个培育品系中的遗传多态性,并分析了多态位点不同基因型与鸡胴体及脂肪性状的相关性。结果发现,在6个供试群体中检测到1个Pst Ⅰ酶切突变位点,测序证实为新发现的鸡PLIN基因2224 bp处G→T突变,该突变位点在供试群体中共检测到两种基因型(A1A1和A1A2),等位基因A1在所有供试群体中均表现为优势等位基因。关联分析结果表明,PLIN基因2224多态位点对鸡胴体性状和脂肪性状影响均不显著(P＞0.05)。多重比较结果表明,A1A1基因型个体在胴体和脂肪性状上总体优于A1A2基因型个体,但各性状均值在两种基因型个体间差异均不显著(P＞0.05)。本研究结果表明,该位点不能作为鸡胴体和脂肪性状的有效分子标记。

Polymorphism in PLIN Gene Intron 5 and its Association with Carcass and Fatness Traits in Chicken

The genetic polymorphisms in perilipin(PLIN) gene were detected by PCR-RFLP method in five Shandong local chickens and one breeding strain including Jining Bairi chicken, Wenshang Luhua chicken et al, the correlations between the SNP loci and the carcass and fatness traits were analyzed. As a result, an G→T mutation of Pst Ⅰ restricton site at 2224 bp in PLIN gene was firstly identified in the six populations. Two genotypes (A1A1 and A1A2) were detected, and the allele A1 was predominant in all the six experimental populations. The statistical analysis showed that the 2224 bp polymorphism locus was not association with carcass and fatness traits (P＞0.05)。The chickens with A1A1 genotype had higher carcass and fatness trait means than chickens with A1A2 genotype, but no significant difference was found between them (P＞0.05). The results of this research showed that the 2224 bp polymorphism locus in chicken PLIN wasn't an effective marker for carcass and fatness traits in chickens.