| 广西大王岭和大明山自然保护区苏云金芽孢杆菌收集与鉴定 |
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| 引用本文： | 谢柳,张文飞,全嘉新,刘卓明,叶大维,李有志,方宣钧.广西大王岭和大明山自然保护区苏云金芽孢杆菌收集与鉴定[J].广西农业生物科学,2009(1):62-68. |
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| 作者姓名： | 谢柳 张文飞 全嘉新 刘卓明 叶大维 李有志 方宣钧 |
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| 作者单位： | 广西大学生命科学与技术学院;海南海德热带农业资源研究所;南开大学生命科学院; |
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| 基金项目： | 中国Bt资源收鉴与利用项目资助 |
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| 摘 要： | 从广西大王岭和大明山两个自然保护区共采集到土样264份，共分离出597株芽孢杆菌，通过光学和电子显微镜检观察，16株分离株观察到伴胞晶体蛋白，初步确定为苏云金芽胞杆菌（Bacillus thuringiensis，简称Bt），出菌率为6．06％。在16株肪分离株中，有4株在芽孢形成过程中能产菱形晶体蛋白，其余12株能产圆形和其他形状的晶体蛋白。利用PCR—RFLP方法和SDS．PAGE方法对16株助分离菌进行了蛋白和基因型的鉴定，结果表明，16株分离株中含有4株crylAc基因，表达约130kD的晶体蛋白，其中含有cry30基因和cry40基因的菌株分别1株和3株，表达大约75kD的晶体蛋白；另外8株戤菌株表达蛋白大小不一，其基因型尚不能确定，有待进一步分析。生物测定表明，产菱形晶体含有crylAc基因的4株励分离株对鳞翅目小菜夜蛾幼虫有很强的毒杀活性，而其它分离株对小菜夜蛾没有毒杀活性。
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| 关 键 词： | 苏云金芽孢杆菌 Bt分离株 杀虫晶体蛋白 SDS-PAGE PCR-RFLP |
Bacillus thuringiensis Collection and Isolates Identification from Damingshan and Dawangling Natural Reserves in Guangxi Province |
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| Xie Liu , Zhang Wenfei , Quan Jiaxin , Liu Zhuoming , Ye Dawei Li Youzhi Fang Xuanjun College of Life Science , Technology,Guangxi University,Nanning, Haide Institute of Tropical Agricultural Resources,Sanya, College of Life Sciences,Nankai University,Tianjin.Bacillus thuringiensis Collection and Isolates Identification from Damingshan and Dawangling Natural Reserves in Guangxi Province[J].Journal of Guangxi Agricultural and Biological Science,2009(1):62-68. |
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| Authors: | Xie Liu Zhang Wenfei Quan Jiaxin Liu Zhuoming Ye Dawei Li Youzhi Fang Xuanjun College of Life Science Technology Guangxi University Nanning Haide Institute of Tropical Agricultural Resources Sanya College of Life Sciences Nankai University Tianjin |
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| Institution: | Xie Liu 1,2 Zhang Wenfei 1,2 Quan Jiaxin 1,2 Liu Zhuoming 1,2 Ye Dawei 3 Li Youzhi 1 Fang Xuanjun 2 1 College of Life Science , Technology,Guangxi University,Nanning,530005,2 Haide Institute of Tropical Agricultural Resources,Sanya,572025,3 College of Life Sciences,Nankai University,Tianjin,300071 |
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| Abstract: | A total of 264 soil samples were collected from Damingshan and Dawangling Natural Reserves in Guangxi Province of southern China. From those 264 soil samples, 597 Bacillus isolates were harvested and among them, 16 isolates were further identified to be Bacillus thuringiesis using oil lens optical microscope and scanning electronic microscope. The isolation rate of these 264 soil samples is 6.06%. Among the 16 B. thuringiensis isolates, four isolates were observed to produce diamond parasporal crystal prote... |
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| Keywords: | Bacillus thuringiensis Bt Isolates ICPs SDS-PAGE PCR-RFLP |
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