| 火龙果总DNA提取方法比较研究 |
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| 引用本文： | 余志雄,欧高政,陈清西,袁亚芳. 火龙果总DNA提取方法比较研究[J]. 中国农学通报, 2010, 26(4): 300-303 |
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| 作者姓名： | 余志雄 欧高政 陈清西 袁亚芳 |
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| 作者单位： | 1. 福建农业职业技术学院,福州,350119
2. 福建农林大学园艺学院,福州,350002 |
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| 基金项目： | 福建省科技厅重点项目"台湾特色水果火龙果引种及关键栽培技术研究" |
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| 摘 要： | 摘要：火龙果组织中含有多糖及其他次生代谢物质，因而难以提取高质量的DNA。本试验以火龙果嫩茎为材料，进行多种DNA提取方法比较研究，并通过琼脂糖凝胶电泳对所提取的DNA样品进行检测，结果表明，改良CTAB法和微量CTAB法均可用于火龙果DNA提取，尤其是微量CTAB法，提取的DNA纯度较为理想，能满足后续分子生物学研究的要求。
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| 关 键 词： | 烤烟 烤烟 品种 产质量 抗性 |
| 收稿时间： | 2009-08-28 |
| 修稿时间： | 2009-12-13 |
Study on Comparison of Methods for Pitaya Total DNA Extraction |
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| Yu Zhixiong,Ou Gaozheng,Chen Qingxi,Yuan Yafang. Study on Comparison of Methods for Pitaya Total DNA Extraction[J]. Chinese Agricultural Science Bulletin, 2010, 26(4): 300-303 |
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| Authors: | Yu Zhixiong Ou Gaozheng Chen Qingxi Yuan Yafang |
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| Affiliation: | Yu Zhixiong1,Ou Gaozheng1,Chen Qingxi2,Yuan Yafang1(1Fujian Vocational College of Agriculture,Fuzhou 350119,2Horticultural Department,Fujian Agricultural , Forestry University,Fuzhou 350002) |
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| Abstract: | The tissues of Pitaya richly contain polysaccharide and other subsequent metabolizing substance,so it is difficult to extract DNA effectively.The study compares different methods for Pitaya DNA extraction on tender stem of Pitaya.Then the purified DNA was detected through Eppendorf Biophotomete.The researches indicated that modified CTAB and micro-CTAB extraction methods were both suitable for extracting DNA of Pitaya,especially the latter.DNA purity gained by those two methods was available to the further ... |
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| Keywords: | Pitaya(Hylocereus undatus Britt.&Rose) total DNA micro-CTAB modified CTAB |
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