| 半定量RT-PCR技术的研究及应用 |
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| 引用本文： | 金凤媚,薛俊,郏艳红,刘仲齐.半定量RT-PCR技术的研究及应用[J].天津农业科学,2008,14(1):10-13. |
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| 作者姓名： | 金凤媚 薛俊 郏艳红 刘仲齐 |
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| 作者单位： | 天津市农业生物技术研究中心,天津,300192 |
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| 摘 要： | 介绍了半定量逆转录多聚合酶链式反应(RT-PCR)技术的原理和实验过程中潜在的问题,并且讨论了该技术在实际中的应用。
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| 关 键 词： | 半定量RT-PCR 反应参数 应用 |
| 文章编号： | 1006-6500（2008）01-0010-04 |
| 收稿时间： | 2007-03-27 |
| 修稿时间： | 2007-12-10 |
Study and Application of Semi-quantitative RT-PCR Technique |
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| JIN Feng-mei,XUE Jun,JIA Yan-hong,LIU Zhong-qi.Study and Application of Semi-quantitative RT-PCR Technique[J].Tianjin Agricultural Sciences,2008,14(1):10-13. |
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| Authors: | JIN Feng-mei XUE Jun JIA Yan-hong LIU Zhong-qi |
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| Abstract: | The semi-quantitative RT-PCR technique is the popular method to study the level of gene express by its simple and sensitiveness. The principles, questions of the method were introduced, and the applications were also discussed. |
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| Keywords: | semi-quantitative RT-PCR reaction parameter application |
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