|
|||||
|
|
| 西瓜品种和育种材料的DNA指纹分析 | |
| 引用本文： | 刘立功,许勇,张雪平,张仁兵,张海英,屈广琪,陈雅萍. 西瓜品种和育种材料的DNA指纹分析[J]. 果树学报, 2004, 21(6): 573-578 |
| 作者姓名： | 刘立功 许勇 张雪平 张仁兵 张海英 屈广琪 陈雅萍 |
| 作者单位： | 1. 国家蔬菜工程技术研究中心,北京,100089 2. 浙江大学园艺系,浙江,杭州,310029 3. 首都师范大学生物系,北京,100089 |
| 基金项目： | 863计划项目2001AA241174，北京市自然科学基金重点项目5991017。 |
| 摘 要： | 利用RAPD技术对国内外26份西瓜材料进行遗传亲缘关系研究、构建每份材料的特异指纹。在720个RAPD随机引物中筛选出15个引物用于聚类分析,将供试26份材料分为6个类群1个美国生态型类群、2个中间生态型类群、1个东亚生态型类群、2个野生类群。将京欣一号杂种纯度鉴定的RAPD特异标记K14-1500和N04-600成功转化为SCAR标记,并应用于京欣一号纯度鉴定实践。 |
| 关 键 词： | 西瓜(Citrulluslanatus) RAPD SCAR DNA指纹 纯度鉴定 |
| 文章编号： | 1009-9980(2004)06-573-06 |
| 修稿时间： | 2004-03-05 |
DNA Fingerprinting Analysis of Varieties and Inbreds in Watermelon |
|
| Liu Ligong,Xu Yong,Zhang Xueping,Zhang Renbing,Zhang Haiying,Qu Guangqi,Chen Yaping. DNA Fingerprinting Analysis of Varieties and Inbreds in Watermelon[J]. Journal of Fruit Science, 2004, 21(6): 573-578 | |
| Authors: | Liu Ligong Xu Yong Zhang Xueping Zhang Renbing Zhang Haiying Qu Guangqi Chen Yaping |
| Affiliation: | Liu Ligong1,Xu Yong 1*,Zhang Xueping1,Zhang Renbing2,Zhang Haiying 1,Qu Guangqi1,and Chen Yaping3 |
| Abstract: | RAPD analysis was applied to assess the genetic relationships among 26 accessions of watermelon [Citrullus lanatus(Thunb.) Mansf.], and to construct fingerprinting of these accessions. Results of clustering analysis based on polymorphism amplified with the fifteen primers which were screened from 720 arbitrary 10-mer primers showed that the 26 accessions were assigned to 6 groups which were 1 East-Asia group, 1 American group, 2 medial groups and 2 African wild groups. RAPD makers, K14-1 500 bp and N04-600 bp were successfully transformed to SCAR markers, and were used for identification of the purity of watermelon hybrid Jingxin №.1. |
| Keywords: | Watermelon[Citrullus lanatus (Thunb.) Mansf.] RAPD SCAR DNA fingerprinting Purity identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |