邓恩桉组培生根影响因子的研究

对耐寒桉树邓恩桉的组培生根问题进行了系统的研究.以7个邓恩桉优良无性系组培苗为试验材料,研究了ABT、IBA、NAA,活性炭,生物素,基本培养基(大量元素,微量元素,有机物),蔗糖,温度,光照及继代次数等对组培苗生根的影响,结果表明,不同无性系的生根率差异非常显著,其中激素种类和浓度、基本培养基种类及其大量元素的含量、蔗糖浓度和继代次数对生根有显著性的影响;光照对生根有一定的影响;活性炭和生物素对生根基本没有作用.组培苗的生根采用两步生根法,在培养基S+IBA 3 mg.L-1+蔗糖2%暗培养9 d后转接到不含激素的培养基上进行光照培养,其中24、25号无性系的生根率达到80%以上,试验取得了较大的突破,基本解决了邓恩桉组培苗难生根的问题.

Studies on effects of different factors on Eucalyptus dunnii in vitro culture

In this paper,the effects of different factors on Eucalyptus dunnii in vitro culture,a cold-resisting species,were systematically studied.The experiment materials were collected from clump shoots of 7 excellent clones of E.dunnii,and the factors included numbers of subculture,IAA,IBA,NAA,AC,VH,basic media,the ingredients of basic media(macroelement,microelement and organic compound),saccharose,tempetature and illumination.The effects were as follows:the difference of rooting rate was very distinct among 7 clones,effects of numbers of subculture,IAA,IBA,NAA,basic media,the ingredients of basic media(macroelement,microelement and organic compound) and that of illumination on rooting were distinct;that of illumination was revetively distinct;that of VC and VH were little.Twostep rooting method was adopted,the efficient seedlings were cultured for 9 d without illumination in rooting media with IBA 3 mg·L~-1 first,and then cultured in rooting media without IBA in natural light.And solving the hard problem of rooting in breeding E.dunnii via vitro culture.The experiment was valued by extending the(utilization) of E.dunnii through asexual propagation.