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| 地高辛标记LEA3蛋白基因探针的制备及其在转基因草莓核酸杂交中的应用 | |
| 引用本文： | 王俊丽,葛会波,彭士琪,张红梅,续九如. 地高辛标记LEA3蛋白基因探针的制备及其在转基因草莓核酸杂交中的应用[J]. 果树学报, 2004, 21(4): 331-333 |
| 作者姓名： | 王俊丽 葛会波 彭士琪 张红梅 续九如 |
| 作者单位： | 1. 河北大学生命科学学院,河北,保定,071002;北京林业大学生物科学与技术学院,北京,100083 2. 河北农业大学园艺学院,河北保定,071000 3. 河北大学生命科学学院,河北,保定,071002 4. 北京林业大学生物科学与技术学院,北京,100083 |
| 基金项目： | 河北省自然科学基金资助项目(302110)。 |
| 摘 要： | 用地高辛对胚胎发生晚期丰富蛋白LEA3基因片段(1.0 kh)进行了探针标记。斑点杂交表明,该探针的检测灵敏度为5 Pg。用地高辛标记和未标记的标准分子量DL 2000进行杂交时,只检测到2.00、1.00、0.75、0.50 kb4条杂交带。应用LEA3探针对转基因草莓(Fragaria ananassa Duch.)进行了Southern blot分析,检测到1.0 kh杂交带,表明外源LEA3基因整合到草莓染色体上。 |
| 关 键 词： | 地高辛 LEA3基因 探针标记 转基因草莓 |
| 文章编号： | 1009-9980(2004)04-331-03 |
| 修稿时间： | 2003-05-10 |
Preparation of LEA3 Protein Gene Probe Labeled by Digoxigenin and Application in Detection of Transgenic Strawberry |
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| Wang Junli,Ge Huibo,Peng Shiqi,Zhang Hongmei,and Xu Jiuru. Preparation of LEA3 Protein Gene Probe Labeled by Digoxigenin and Application in Detection of Transgenic Strawberry[J]. Journal of Fruit Science, 2004, 21(4): 331-333 | |
| Authors: | Wang Junli Ge Huibo Peng Shiqi Zhang Hongmei and Xu Jiuru |
| Abstract: | Plasmid DNA probe of late embryogenesis abundant protein gene, LEA3 (1.0 kb), was labeled by digoxigenin. Dot blot showed that the probe was sensitive, able to detect 5 pg homologous DNA. Four hybridization bands (2.0 kb, 1.0 kb, 0.75 kb and 0.5 kb) were detected in Southern blot analysis of marker DL2000. The LEA3 probe labeled by digoxigenin was used in Southern blot analysis of transgenic strawberry (Fragaria ananassa Duch.) and hybridization bands were detected. The result indicated that LEA3 gene was integrated into the genome of strawberry. |
| Keywords: | Digoxigenin LEA3 gene Probe label Transgenic strawberry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |