HPLC法同时测定虎杖中多组分的含量 |
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引用本文: | 张明昶,彭小冰,王玉明,邵进明.HPLC法同时测定虎杖中多组分的含量[J].安徽农业科学,2010,38(24):13086-13087,13089. |
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作者姓名: | 张明昶 彭小冰 王玉明 邵进明 |
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作者单位: | 贵阳中医学院,贵州贵阳,550002;贵阳中医学院,贵州贵阳,550002;贵阳中医学院,贵州贵阳,550002;贵阳中医学院,贵州贵阳,550002 |
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摘 要: | 目的]建立了同时测定虎杖(Polygonum cuspidatum Sieb.et Zucc.)提取物中白藜芦醇、虎杖苷、大黄素和大黄素甲醚含量的HPLC法。方法]采用DIKMAC18色谱柱(200.0mm×4.6mm,5μm),以乙腈-0.1%磷酸溶液为流动相,流速为1.0ml/min,波长为303nm,柱温为30℃,进样量为10μl。结果]白藜芦醇、虎杖苷、大黄素和大黄素甲醚对应的回归方程分别为y=7116169x+15631(r=0.9999)、y=395313x+50(r=0.9999)、y=2752869x-2664(r=0.9999)、y=463886x-1413(r=0.9999),且白藜芦醇在0.026~0.520μg范围内、虎杖苷在0.045~0.765μg范围内、大黄素在0.026~0.520μg范围内、大黄素甲醚在0.026~0.442μg范围内线性关系良好。结论]该研究建立的方法可行,重复性好,可用于虎杖药材的质量控制。
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关 键 词: | 虎杖 白藜芦醇 虎杖苷 大黄素 大黄素甲醚 HPLC |
Content Determination of Multiple Constituents in Polygonum cuspidatum Sieb.et Zucc. Simultaneously by HPLC |
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Institution: | ZHANG Ming-chang et al (Guiyang College of Traditional Chinese Medicine,Guiyang ,Guizhou 550002) |
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Abstract: | Objective] The research aimed to establish a method for the content determination of resveratrol,polydatin,emodin and physcion in Polygonum cuspidatum Sieb.et Zucc. simultaneously by HPLC. Method] HPLC method was established with DIKMA C18 column(200.0 mm×4.6 mm,5 μm)and a mobile phase of acetonitrile-0.1% phosphoric acid. The flow rate was 1.0 ml/min,the detection wavelength was 303 nm and the column temperature was 30 ℃. Result] The regression equations of resveratrol,polydatin,emodin and physcion were y=7 116 169 x + 15 631(r=0.999 88),y=395 313 x + 50,y =2 752 869 x -2 664 and y = 463 866 x -1 413 respectively. Resveratrol,polydatin,emodin and physcion showed a good linear relationship in the range of 0.026-0.520,0.045-0.765,0.026-0.520 and 0.026-0.442 μg respectively. Conclusion] The established method is feasible and the repeatability is good. The method can be used for quality control of P. cuspidatum medicine materials. |
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Keywords: | HPLC |
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