| 波棱瓜叶片愈伤组织诱导研究初报 |
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| 引用本文： | 王宏霞,陈垣,郭凤霞,蔡子平,柳福智.波棱瓜叶片愈伤组织诱导研究初报[J].甘肃农业大学学报,2008,43(4). |
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| 作者姓名： | 王宏霞 陈垣 郭凤霞 蔡子平 柳福智 |
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| 作者单位： | 1. 甘肃农业大学农学院,甘肃,兰州,730070
2. 甘肃农业大学生命科学技术学院,甘肃,兰州,730070 |
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| 基金项目： | 甘肃省科技攻关项目，甘肃省科技扶贫项目，甘肃省自然科学基金，甘肃省兰州市科技局科研项目 |
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| 摘 要： | 通过正交试验研究了6-BA、2,4-D和NAA不同激素配比的MS培养基对波棱瓜叶片愈伤组织形成的影响.结果表明,2,4-D对波棱瓜叶片愈伤组织形成的作用最显著,其次为6-BA和NAA.诱导愈伤组织的最佳培养基为:MS+2.0 mg/L 6-BA+2.0 mg/L 2,4-D+0.5 mg/L NAA和MS+2.0 mg/L 6-BA+1.5 mg/L 2,4-D+0.5 mg/L NAA,这两种培养基诱导率均达到90%.
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| 关 键 词： | 波棱瓜 愈伤组织 正交设计 叶片 激素 |
Primary report on induction of leave callus of Tibet Herpetospermum caudigerum Wall |
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| WANG Hong-xia,CHEN Yuan,GUO Feng-xia,CAI Zi-ping,LIU Fu-zhi.Primary report on induction of leave callus of Tibet Herpetospermum caudigerum Wall[J].Journal of Gansu Agricultural University,2008,43(4). |
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| Authors: | WANG Hong-xia CHEN Yuan GUO Feng-xia CAI Zi-ping LIU Fu-zhi |
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| Abstract: | The calli induction from leaves of Tibet Herpetospermum caudigerum were studied through orthogonal test.The results showed that 2,4-D has a significant effect on callus information,then followed by 6-BA and NAA.The two best medium for callus induction were MS 2.0 mg/L 6-BA 2.0 mg/L 2,4-D 0.5 mg/L NAA and MS 2.0 mg/L 6-BA 1.5 mg/L2,4-D 0.5 mg/L NAA,the induction rate of the two medium could reach 90 %. |
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| Keywords: | Herpetospermum caudigerum Wall callus orthogoal design leaves auxin |
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