A new powdery mildew resistance gene: Introgression from wild emmer into common wheat and RFLP-based mapping |
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Authors: | JK Rong E Millet J Manisterski M Feldman |
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Institution: | (1) Department of Plant Sciences, The Weizmann Institute of Science, Rehovot, 76100, Israel;(2) Institute of Cereal Crops Improvement, Tel Aviv University, Tel Aviv, Israel |
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Abstract: | An Israeli accession (TTD140) of wild emmer, Triticum turgidum var. dicoccoides, was found resistant to several races of powdery mildew. Inoculation of the chromosome-arm substitution lines (CASLs) of
TTD140, in the background of the Israeli common wheat cultivar ‘Bethlehem’ (BL), with five isolates of powdery mildew revealed
that only the line carrying the short arm of chromosome 2B of wild emmer (CASL 2BS) exhibited complete resistance to four
of the five isolates. To map and tag the powdery mildew resistance gene, 41 recombinant substitution lines, derived from a
cross between BL and CASL 2BS, were used to construct a linkage map at the gene region. The map, which encompasses 69.5 cM
of the distal region of chromosome arm 2BS, contains six RFLP markers, a morphological marker (glaucousness inhibitor, W1
I), and the powdery mildew resistance gene. Segregation ratios for resistance in F2 of BL × CASL 2BS and in the recombinant lines, combined with the susceptability of F1 progeny to all tested isolates, indicate that resistance is controlled by a single recessive allele. This alleleco-segregated
with a polymorphic locus detected by the DNA marker Xwg516, 49.4 cM from the terminal marker Xcdo456. The new powdery mildew resistance gene was designated Pm26.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Powdery mildew resistance gene RFLP-based mapping substitution lines Triticum wild wheat |
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