| 乙草胺人工抗原的合成及其单克隆抗体的制备 |
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| 引用本文： | 梅平,惠小敏,王雄. 乙草胺人工抗原的合成及其单克隆抗体的制备[J]. 农药学学报, 2008, 10(1): 109-112 |
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| 作者姓名： | 梅平 惠小敏 王雄 |
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| 作者单位： | 长江大学,化学与环境工程学院,湖北,荆州,434023;长江大学,化学与环境工程学院,湖北,荆州,434023;北京中检维康技术有限公司,北京,100044;北京中检维康技术有限公司,北京,100044 |
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| 摘 要： | 通过乙草胺与3-巯基丙酸在碱性条件下反应合成了半抗原——乙草胺-巯基丙酸(AMPA)。采用活性酯法将半抗原AMPA分别与牛血清白蛋白(BSA)和卵清蛋白(OVA)偶联制备了人工抗原。通过紫外光谱测定了AMPA与BSA及OVA间的分子结合比分别为41和26。用AMPA-BSA免疫小鼠,制备得到的单克隆抗体的效价为1∶ 16×104。以AMPA-OVA作包被抗原,用乙草胺单克隆抗体建立了ELISA检测方法,IC50值为0.55 μg/L,方法的检测范围为0.04~5 μg/L,检测限为0.04 μg/L。
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| 关 键 词： | 乙草胺 半抗原 人工抗原 单克隆抗体 |
| 文章编号： | 1008-7303(2008)01-0109-04 |
| 收稿时间： | 2007-10-22 |
| 修稿时间： | 2007-10-02 |
Synthesis of Artificial Antigen and Preparation of Monoclonal Antibody for Acetochlor |
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| MEI Ping,HUI Xiao-Min and WANG Xiong. Synthesis of Artificial Antigen and Preparation of Monoclonal Antibody for Acetochlor[J]. Chinese Journal of Pesticide Science, 2008, 10(1): 109-112 |
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| Authors: | MEI Ping HUI Xiao-Min WANG Xiong |
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| Affiliation: | 1.College of Chemistry and Environmental Engineering of Yangtze University,Jingzhou 434023,China2.Clover Technology Group Inc.of Beijing,Beijing 100044,China |
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| Abstract: | The hapten of acetochlor-MPA(AMPA) was synthesized by the reaction of acetochlor with 3-mercaptopropionic acid in alkaline solution.The artificial antigens (AMAP-BSA/OVA) were synthesized by coupling of molecules between hapten and BSA,OVA respectively with active ester method.The molar ratio was 41,26 for AMPA-BSA and AMPA-OVA respectively detected by UV spectro-photometer.Mice were immunized by injection of antigen AMPA-BSA.The monoclonal antibody was produced with titer of 1∶ 16×104.The ELISA method based upon AMPA-OVA and monoclonal antibody was established.This ELISA method showed its IC50 value was 0.55 μg/ L,the response range for acetochlor was 0.04~5 μg/L,and limit of detection was 0.04 μg/L. |
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| Keywords: | acetochlor hapten artificial antigen monoclonal antibody |
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