| 建昌鸭线粒体基因组全序列分析 |
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| 引用本文： | 涂剑锋,黄银花,刘三凤,李宁. 建昌鸭线粒体基因组全序列分析[J]. 西南大学学报(自然科学版), 2010, 32(2) |
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| 作者姓名： | 涂剑锋 黄银花 刘三凤 李宁 |
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| 作者单位： | 中国农业大学农业生物技术国家重点实验室;中国农业科学院特产研究所;江西农业大学动物科技学院; |
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| 基金项目： | 国家自然科学基金资助项目(30221005); 国家重点基础研究发展计划(2006CB200100) |
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| 摘 要： | 参照近源物种线粒体基因组序列设计引物15对,用PCR产物直接测序法测得建昌鸭(Anas platyrhychos)线粒体基因组全序列,初步分析其特点和各基因的定位.结果显示:建昌鸭线粒体基因组全长16 606 bp,碱基A、T、G、C的含量分别为29.21%、22.19%、15.77%、32.83%,包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码调控区(D-loop),基因组结构和已知雁形目鸟类的完全相同.基于线粒体13个蛋白质编码基因序列,采用邻接法构建雁形目8个物种的系统进化树,所得结果与传统的系统分类基本一致.
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| 关 键 词： | 建昌鸭 线粒体基因组 序列分析 |
Complete Mitochondrial Genome Sequence Analysis of Jianchang Duck |
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| TU Jian-feng,,HUANG Yin-hua,LIU San-feng,LI Ning.State Key Laboratory for Agrobiotechnology,China Agricultural University,Beijing ,China. Complete Mitochondrial Genome Sequence Analysis of Jianchang Duck[J]. Journal of southwest university (Natural science edition), 2010, 32(2) |
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| Authors: | TU Jian-feng HUANG Yin-hua LIU San-feng LI Ning.State Key Laboratory for Agrobiotechnology China Agricultural University Beijing China |
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| Affiliation: | TU Jian-feng1,2,HUANG Yin-hua1,LIU San-feng3,LI Ning11.State Key Laboratory for Agrobiotechnology,China Agricultural University,Beijing 100094,China,2.Institute of Special Wild Economic Animal , Plant Science,Chinese Academy of Agricultural Science,Jilin Jilin 132109,3.College of Animal Science , Technology,Jiangxi Agricultural University,Nanchang 330045 |
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| Abstract: | The complete mitochondrial genome of Jianchang duck(Anas platyrhychos) was determined by direct DNA sequencing based on the PCR products amplified with 15 pairs of primers designed on related species.The entire genome was found to be 16 606 bp in length and contain 37 genes(13 protein coding genes,2 rRNA and 22 tRNA) and a non-coding control region(D-loop).The content of the nucleotides A,T,G and C was 29.19%,22.20%,15.80% and 32.81%,respectively.The characteristics of the mtDNA of Jianchang duck appeared t... |
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| Keywords: | Jianchang duck mitochondrial genome sequence analysis |
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