高效液相色谱法测定苹果果皮的类胡萝卜素

用高效液相色谱法测定苹果果皮多种类胡萝卜素。以丙酮与石油醚提取色素 ,用NovapakC18(4 .6mm×15 0mm)色谱柱 ,乙腈∶甲醇∶二氯甲烷 (70∶2 0∶10 )流动相 ,在 45 0nm下检测类胡萝卜素。结果表明 ,玉米黄质、叶黄素、番茄红素与 β 胡萝卜素的回收率分别是94.2 7%～ 99.82 %、84.5 6 %～ 89.41%、87.5 3～ 93.5 4%与 88.0 8%～93 .2 4%。其中富士果仅检测到玉米黄质 (5 .9× 10 -3 μg/mm2 )与 β 胡萝卜素 (5 .4× 10 -3 μg/mm2 ) ;蛇果 (红星 )只有玉米黄质 (9.1× 10 -3 μg/mm2 )。

The Determination of Carotenoids in Apple Peel by HPLC

A reversed-phase high performance liquid chromatographic method was used to determine carotenoids in apple peel.Carotenoids were extracted from apple peel with acetone and petroleum ether.Using Novapak C18 column(4.6 mm×150 mm),acetonitrile∶methanol∶methylene chloride (70∶20∶10) as mobile phase,carotenoids were eluted and monitored at 450 nm.It was shown that recoveries of zeaxanthin,lutein,lycopene and β-carotene were 94.27%～99.82%,84.56%～89.41%，87.53%～93.54%，and 88.08%～93.24%.‘Fuji’ apple contained zeaxanthin (5.9×10－3 μg/mm2) and β-carotene (5.4×10－3 μg/mm2) and Red Delicious apple only zeaxanthin (9.1×10－3 μg/mm2).