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| 大肠杆菌菌体氨基酸组成对高渗透环境的响应 | |
| 引用本文： | 申铁,杨琦,郭玉双,乙引,文汉,芮斌.大肠杆菌菌体氨基酸组成对高渗透环境的响应[J].江西农业学报,2012,24(12):140-143. |
| 作者姓名： | 申铁 杨琦 郭玉双 乙引 文汉 芮斌 |
| 作者单位： | 1. 贵州师范大学生命科学学院、贵州省植物生理与发育重点实验室,贵州贵阳,550001 2. 安徽农业大学生命科学学院,安徽合肥,230036 3. 贵州省烟草科学研究所,贵州贵阳,550001 |
| 摘 要： | 在正常和高渗透压(高盐)环境下培养大肠杆菌,利用气相色谱质谱联用仪(GC-MS)对大肠杆菌代谢物中的氨基酸进行了定性定量分析。结果表明:在两组中均鉴定出14种氨基酸,且两组的氨基酸种类相同;与对照组中同种氨基酸的相对含量相比,实验组中脯氨酸和甲硫氨酸的相对含量明显降低,丝氨酸、苏氨酸、苯丙氨酸、天冬氨酸、鸟氨酸、赖氨酸和组氨酸的相对含量明显增加,而丙氨酸、甘氨酸、缬氨酸、亮氨酸、谷氨酸的相对含量与对照组相近。 |
| 关 键 词： | 高渗透环境 大肠杆菌 GC-MS 氨基酸分析 代谢组学 |
Response of Amino Acid Compositions in Escherichia coil to Highly-osmotic Environment |
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| SHEN Tie , YANG Qi , GUO Yu-shuang , YI Yin , WEN Han , RUI Bin.Response of Amino Acid Compositions in Escherichia coil to Highly-osmotic Environment[J].Acta Agriculturae Jiangxi,2012,24(12):140-143. | |
| Authors: | SHEN Tie YANG Qi GUO Yu-shuang YI Yin WEN Han RUI Bin |
| Institution: | 1.College of Life Science,Guizhou Normal University;Key Laboratory of Plant Physiology and Development Regulation of Guizhou Province,Guiyang 550001,China;2.School of Life Science,Anhui Agricultural University,Hefei 230026,China;3.Guizhou Institute of Tobacco Science,Guiyang 550001,China) |
| Abstract: | In the study,the qualitative and quantitative analyses of amino acid compositions in Escherichia coli metabolites in natural and highly-osmotic stress conditions were done by using gas chromatography mass spectrometry(GC-MS).The results showed that 14 kinds of amino acids were identified in both the test group and control group.Comparing the relative content of each amino acid in the two groups,the relative content of proline and methionine in test group significantly reduced,and that of serine,threonine,phenylalanine,aspartic acid,ornithine,lysine and histidine in test group significantly increased,while the relative content of alanine,glycin,valine,leucine and glutamic acid in test group was almost the same as that in the control group. |
| Keywords: | Highly-osmotic environment Escherichia coli GC-MS Analysis of amino acids Metabonomics |
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