鱿鱼肝脏活性肽的制备及生物活性研究

目的]为鱿鱼肝脏的高值化利用提供基础研究数据。方法]以鱿鱼肝脏蛋白为原料,通过蛋白酶水解来制备生物活性肽,研究了多种蛋白酶水解鱿鱼肝脏制得水解液的抗凝血活性、抑制血管紧张素Ⅰ转换酶（ACE）活性及抗氧化活性。结果]当鱿鱼肝脏水解液浓度为5 mg/ml时,中性蛋白酶的水解液对ACE抑制率为68.0%;APTT值为59.8 s,TT值为34.2 s,比空白对照组分别延长26.7和20.6 s,表明鱿鱼肝脏的中性蛋白酶水解液具有抗凝血活性。中性蛋白酶解液对Fe3＋的还原力为0.588,羟自由基清除能力为64.5%,说明鱿鱼肝脏中性蛋白酶水解液具有较好的抗氧化活性;而其余的木瓜蛋白酶、酸性蛋白酶、碱性蛋白酶、胰蛋白酶的鱿鱼肝脏水解液的抗凝血活性和ACE抑制活性均低于中性蛋白酶水解液的酶解液活性。结论]鱿鱼肝脏蛋白质具有较好的综合利用价值。

Preparation of Bioactive Peptides from Squid Liver and Its Bioactivity Study

Objective] The research aimed to provide basic research data for high-value utilization of squid liver. Method]Taking protein of squid liver as materials,the bioactive peptide was prepared by protease hydrolysis. The anticoagulant activity,inhibitory activity and antioxidant activity of angiotensin converting enzyme-Ⅰ of the squid liver hydrolyzed with many kinds of protease were studied. Result] When the hydrolyzate concentration of squid liver was 5 mg / ml,the inhibition rate of neutral protease hydrolyzate to ACE was 68. 0%. APTT and TT value was 59. 8 and 34. 2 s respectively,which were 26. 7 s and 20. 6 s longer than the blank control respectively. This indicated that the neutral protease hydrolyzate of squid liver had anticoagulant activity. The reducing power of neutral protease hydrolyzate to Fe3 ＋was 0. 588,the scavenging capacity of hydorxyl radical was 64. 5%,which indicated neutral protease hydrolyzate of squid liver had higher antioxidant activity. The anticoagulant activity and ACE inhibitory activity of hydrolyzate of squid liver by papain,acid protease,alkaline protease and trypsase were lower than the activity of neutral protease hydrolyzate. Conclusion] Squid liver protein had better comprehensive utilization value.