| 速生型刺槐遗传转化体系的建立 |
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| 引用本文： | 沈俊岭,赵芳,李云,陈受宜,田秀红.速生型刺槐遗传转化体系的建立[J].核农学报,2006,20(6):477-481. |
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| 作者姓名： | 沈俊岭 赵芳 李云 陈受宜 田秀红 |
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| 作者单位： | 1. 北京林业大学林木花卉遗传育种教育部重点实验室,北京,100083
2. 中国科学院遗传与发育生物研究所,北京,100101 |
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| 基金项目： | 国家林业局科技推广项目 |
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| 摘 要： | 为获得稳定的转基因刺槐植株,以速生型刺槐叶轴为转化受体,对转化过程中的一些影响因素进行了探究,建立了较为完善的遗传转化体系。结果表明:继代20~35d之间刺槐叶轴最适宜转化;卡那霉素的筛选浓度为30mg/L;头孢霉素的抑菌浓度为200mg/L;合适的侵染时间是20min;30mg/L的乙酰丁香酮有利于抗性芽的发生;共培养2d对转化适宜;抑菌7d后再加选择压有利于抗性芽的产生。最后得到的抗性植株经PCR检测,初步证明外源目的基因OsDREB1已整合到刺槐基因组中。
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| 关 键 词： | 刺槐 叶轴 农杆菌 遗传转化 |
| 文章编号： | 1000-8551(2006)06-477-05 |
| 收稿时间： | 05 30 2006 12:00AM |
| 修稿时间： | 2006年5月30日 |
ESTABLISHMENT OF GENETIC TRANSFORMATION SYSTEM OF FAST_GROWING BLACK LOCUST |
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| SHEN Jun-ling,ZHAO fang,LI Yun,CHEN Shou-yi,TIAN Xiu-hong.ESTABLISHMENT OF GENETIC TRANSFORMATION SYSTEM OF FAST_GROWING BLACK LOCUST[J].Acta Agriculturae Nucleatae Sinica,2006,20(6):477-481. |
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| Authors: | SHEN Jun-ling ZHAO fang LI Yun CHEN Shou-yi TIAN Xiu-hong |
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| Institution: | 1. Key Lab of Genetics and Breeding of Forest Tree and Ornamental Plants of Ministry of Education , Beijing Forestry University, Beijing 100083;2.Institute of Genetics and Developmental Biology , Chinese Academy of Science, Beijing 100101 |
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| Abstract: | The rachis of fast-growing Black Locust was used as explants in the study.The factors that affected transformation ratio were studied,and a stable transformation system was established.The results suggested that germinated shoots which 20～35dsdd in vitro were suitable for transgenic.The optimal concentration of kanamycim to the rachis was 30mg/L and that of cefotaxine was 200mg/L.The ideal time for rachis dipping in Agrobacterium was 20min and optimal co-cultivation was 2 days.30mg/L acetosyringone could increase the transformation ratio.The selection of the inoculated rachis was done at 7~(th) days of bacterial inhibiting treatment by cefotaxine.The PCR analysis showed that the target gene OsDREB1 was integrated into the genome of black locust. |
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| Keywords: | black locust rachis agrobacterium genetic transformation |
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