| ISSR Analysis on Genetic Diversity of Local Varieties of Chinese Hu mulberry（Morus L.） |
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| 作者姓名： | WUXIANG Dan-ping ZHANG Lin PAN Gang PAN Yi-le |
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| 作者单位： | [1]College of Biology and Environment, Jiangsu University of Science and Technology, Zhenjiang 212018 [2]The Sericulture Research Insti-tute of Chinese Agricultural Science Academy, Zhenjiang 212018 |
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| 基金项目： | Supported by National Science and Technology Infrastructure（2005DKA21002-09） |
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| 摘 要： | Objective] The aim was to study on the genetic diversity of local varieties of Chinese Hu mulberry （Morus L.）. Method] The genetic diversity of 141 copies of Hu mulberry varieties was analyzed by ISSR molecular markers. Result] 12 ISSR primers had amplified a total of 90 amplified,of which 57 bands were polymorphic,and the polymorphic rate was 63.33%. The genetic similarity coefficients of 141 Hu mulberry germplasm resources varied from 0.633 3 to 1.000 0 with the average of 0.483 35,indicating that there was difference on genetic diversity among different varieties of Hu mulberries. A dendrogram of all 141 Hu mulberry varieties based on the genetic similarity coefficients using ISSR molecular markers was generated by UPGMA cluster method. Clustering of the 141 Hu mulberry varieties did not correspond with the conventional classification involving differences in style,leaf,branch,fruit and other morphological or agronomical characters. Conclusion] Four subgroups clearly represented the genetic relationships in the 141 accessions which were benefit for the variety improvement and germplasm resource conservation.
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| 关 键 词： | 遗传多样性 ISSR分析 桑树品种 地方品种 UPGMA聚类分析 ISSR分子标记 遗传相似系数 种质资源保护 |
ISSR Analysis on Genetic Diversity of Local Varieties of Chinese Hu mulberry (Morus L.) |
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| WUXIANG Dan-ping,ZHANG Lin,PAN Gang,PAN Yi-le.ISSR Analysis on Genetic Diversity of Local Varieties of Chinese Hu mulberry (Morus L.)[J].Agricultural Science ＆ Technology,2010(8):67-72. |
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| Authors: | WUXIANG Dan-ping ZHANG Lin PAN Gang PAN Yi-le College of Biology and Environment Jiangsu University of Science and Technology Zhenjiang ; |
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| Institution: | College of Biology and Environment,Jiangsu University of Science and Technology,Zhenjiang 212018; 2. The Sericulture Research Institute of Chinese Agricultural Science Academy,Zhenjiang 212018 |
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| Abstract: | Objective] The aim was to study on the genetic diversity of local varieties of Chinese Hu mulberry (Morus L.). Method] The genetic diversity of 141 copies of Hu mulberry varieties was analyzed by ISSR molecular markers. Result] 12 ISSR primers had amplified a total of 90 amplified,of which 57 bands were polymorphic,and the polymorphic rate was 63.33%. The genetic similarity coefficients of 141 Hu mulberry germplasm resources varied from 0.633 3 to 1.000 0 with the average of 0.483 35,indicating that there was difference on genetic diversity among different varieties of Hu mulberries. A dendrogram of all 141 Hu mulberry varieties based on the genetic similarity coefficients using ISSR molecular markers was generated by UPGMA cluster method. Clustering of the 141 Hu mulberry varieties did not correspond with the conventional classification involving differences in style,leaf,branch,fruit and other morphological or agronomical characters. Conclusion] Four subgroups clearly represented the genetic relationships in the 141 accessions which were benefit for the variety improvement and germplasm resource conservation. |
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| Keywords: | Germplasm resource Hu mulberry ISSR Genetic diversity |
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