| 利用DNA池技术提高基于InDel标记的种子纯度鉴定效率 |
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| 引用本文： | 兰青阔,余景会,赵新,王永,张桂华,朱珠,陈锐,李欧静,郭永泽,程奕. 利用DNA池技术提高基于InDel标记的种子纯度鉴定效率[J]. 种子, 2012, 31(2): 31-33,38 |
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| 作者姓名： | 兰青阔 余景会 赵新 王永 张桂华 朱珠 陈锐 李欧静 郭永泽 程奕 |
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| 作者单位： | 1. 天津市农业科学院中心实验室,天津,300381
2. 天津科润农业科技股份有限公司黄瓜研究所,天津,300192 |
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| 基金项目： | 天津市应用基础及前沿技术研究计划(项目编号:10 JCYBJC09300);天津市科技支撑项目(项目编号:10 ZCGYNC00400) |
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| 摘 要： | 为提高基于InDel-Pyrosequencing的黄瓜杂交种纯度检测通量,降低检测成本,本研究模拟10种DNA Pooling进行PCR、Pyrosequencing及等位基因频率分析。通过TTest分析不同Pooling间等位基因频率的差异性,确定3 Pooling为种子纯度检测最适Pooling数;建立3 Pooling-InDel-Pyrosequencing标准曲线,其R2达0.999 1;根据该标准曲线,检测黄瓜杂交品种"园中王"30粒杂交种种子纯度,结果为96.67%。本研究丰富了基于InDel-Pyrosequencing的黄瓜杂交种纯度检测技术体系。
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| 关 键 词： | DNA池 InDel标记 焦磷酸测序技术 黄瓜 种子纯度鉴定 |
Improving Seed Purity Identification on InDel Pyrosequencing by DNA Pooling Technology |
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| LAN Qing-kuo , YU Jing-hui , ZHAO Xin , WANG Yong , ZHANG Gui-hua , ZHU Zhu , CHEN Rui , LI Ou-jing , GUO Yong-ze , CHENG Yi. Improving Seed Purity Identification on InDel Pyrosequencing by DNA Pooling Technology[J]. Seed, 2012, 31(2): 31-33,38 |
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| Authors: | LAN Qing-kuo YU Jing-hui ZHAO Xin WANG Yong ZHANG Gui-hua ZHU Zhu CHEN Rui LI Ou-jing GUO Yong-ze CHENG Yi |
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| Affiliation: | 1(1.Central Lab of Tianjin Agriculture Academy Sciences,Tianjin 300381,China; 2.Tianjin Kernel Cucumber Research Institute of Tianjin Agriculture Academy of Science,Tianjin 300192,China) |
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| Abstract: | In order to reduce the seed purity identification cost,DNA pooling technology was combined with InDel-Pyrosequencing to improve the seed purity identification efficiency.The author simulated 10 DNA poolings for PCR,pyrosequencing.The result indicated that 3 pooling was suitable for seed purity identification.And 3 pooling-InDel-Pyrosequencing standard curve and its R2=0.999 1 were builded.Seed purity of Yuan Zhongwang were tested by 3 pooling-InDel-Pyrosequencing based on the standard curve,and the result was 96.67%.The study enriched the seed purity identification method based on InDel-Pyrosequencing. |
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| Keywords: | DNA pooling InDel pyrosequencing cucumber seed purity identification |
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