| 应用原生质体融合技术获得茄子种间体细胞杂种 |
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| 引用本文： | 连勇,刘富中,冯东昕,宋燕,陈钰辉,张松林.应用原生质体融合技术获得茄子种间体细胞杂种[J].园艺学报,2004,31(1):39-42. |
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| 作者姓名： | 连勇 刘富中 冯东昕 宋燕 陈钰辉 张松林 |
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| 作者单位： | ( 中国农业科学院蔬菜花卉研究所, 北京100081; 法国巴黎11 大学植物形态与发育实验室, 法国ORSAY CEDEXBAT. 360, 91405) |
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| 基金项目： | 国家‘8 63’资助项目 ( 2 0 0 2AA2 44 0 11-1)，欧盟资助项目 (ICA4 CT 2 0 0 1 10 0 64 ) |
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| 摘 要： | 应用原生质体电融合技术, 获得了茄子近缘野生种Solanum torvum、S. aethiopicum 与栽培种S.melongena (六叶茄, Dourga) 的种间体细胞融合四倍体再生植株。染色体检测表明该植株为四倍体(2n =4x = 48) , 荧光免疫检测证明为种间体细胞杂种, 青枯病抗性鉴定表明带有抗病基因, 田间生长表现为倾向野生亲本的中间类型。
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| 关 键 词： | 茄子 原生质体 融合 体细胞杂种 |
| 文章编号： | 0513-353X(2004)01-0039-04 |
| 收稿时间： | 2002-12-24 |
| 修稿时间： | 2003-7-25 |
Using Protoplast Electrofusion Technology to Obtain Interspecific Somatic Fusion Hybrid of Eggplant |
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| Lian Yong ,Liu Fuzhong ,Feng Dongxin ,Song Yan ,Chen Yuhui ,Zhang Songlin ,and Darasinh Sihachakr.Using Protoplast Electrofusion Technology to Obtain Interspecific Somatic Fusion Hybrid of Eggplant[J].Acta Horticulturae Sinica,2004,31(1):39-42. |
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| Authors: | Lian Yong Liu Fuzhong Feng Dongxin Song Yan Chen Yuhui Zhang Songlin and Darasinh Sihachakr |
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| Institution: | ( Institute of Vegetables and Flowers , Chinese Academy of Agricultural Sciences, Beijing 100081, China; Morphogenese Vegetale Experimentale, Universite Paris Sud, Bat. 360, 91405 orsay cedex, France) |
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| Abstract: | We obtained regenerated tetroaploid plants from the protoplast electrofusion between Solanum melongena(Dourga)and S.torvum or S.aethiopicum.The ploid level was confirmed by chromosome detection.Fluorescent immunoassay proved that the plants were regenerated from interspecific somatic fusion.Inoculation with Pseudomonas solanacearum indicated that the somatic hybrids carried the resistance gene from the wild relatives.The somatic hybrids were more like the wild relatives in the open field. |
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| Keywords: | Eggplant Protoplast Fusion Somatic hybrid |
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