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野生铁皮石斛内生菌的分离及促生作用研究
引用本文:俞婕,赵凯鹏,董飞,程李梅,肖赛琼,求东妹,胡秀芳.野生铁皮石斛内生菌的分离及促生作用研究[J].现代农业科技,2010(9):96-97.
作者姓名:俞婕  赵凯鹏  董飞  程李梅  肖赛琼  求东妹  胡秀芳
作者单位:浙江理工大学生物工程研究所,浙江杭州,310018
基金项目:浙江省2009年度"新苗人才计划"高校学生科研项目 
摘    要:试验从野生铁皮石斛根、茎中分离获得内生细菌10株、内生真菌21株。以菌液制剂分析其对组培铁皮石斛的促生作用,经筛选及验证,结果表明:内生细菌ZJSH1对铁皮石斛组培苗具有显著的促生作用,且培养液稀释102倍后促生效果最佳。

关 键 词:铁皮石斛  内生菌  分离  促生作用

Separation and Growth-promoting Study of Endophyte in Wild Dendrobium candidum
Authors:YU Jie  ZHAO Kai-peng  DONG Fei  CHENG Li-mei  XIAO Sai-qiong  QIU Dong-mei  HU Xiu-fang
Institution:YU Jie ZHAO Kai-peng DONG Fei CHENG Li-mei XIAO Sai-qiong QIU Dong-mei HU Xiu-fang (Institutes of Bioengineering,Zhejiang Sci-Tech University,Hangzhou zhejiang 310018)
Abstract:Plant endophyte strains were isolated from roots and stems of Dendrobium candidum,10 of them was bacterium,and 21 was fungi. Growth-promoting test was carried out through inoculating endophyte suspension into seedlings of plant tissue culture. The results indicated that ZJSH1 could promote the growth of Dendrobium candidum apparentely,and the best effect was abtained with nutrient solution diluted 100 times.
Keywords:Dendrobium candidum  endophyte  separation  growth-promoting  
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