A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding |
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| Authors: | Li Liu Aili Wang Rudi Appels Junhong Ma Xianchun Xia Ping Lan Zhonghu He Frank Bekes Yueming Yan Wujun Ma |
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| Affiliation: | 1. Western Australian Department of Agriculture and Food, Molecular Plant Breeding CRC, State Agriculture Biotechnology Centre, Murdoch University, Perth WA 6150, Australia;2. Institute of Crop Science, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China;3. Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing 100037, China;4. CIMMYT-China Office, c/o CAAS, Beijing 100081, China;5. CSIRO Plant Industry, Canberra, ACT, Australia;6. Centre for Comparative Genomics, Murdoch University, Perth WA 6150, Australia |
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| Abstract: | High molecular weight glutenin subunits play an important role in determining wheat dough quality as they confer visco-elastic properties to the dough required for mixing and baking performance. In this work, a collection of 103 genotypes of common wheat from 12 countries was used to analyse the composition of HMW-GS by SDS-PAGE and MALDI-TOF-MS. Results indicated that MALDI-TOF technology is suitable for analyzing most HMW-GS alleles. The allelic diversity at Glu-B1 locus include subunits 6+8b*, 7, 7+8, 7+8a*, 7b*+8, 7OE, 7OE+8, 7OE+8a*, 7OE+8b*, 7+9, 13+16, 14+15, 17+18 and 20. The rapid identification of HMW-GS capability of MALDI-TOF-MS is discussed in relation to its value for screening lines in wheat breeding programs, especially in discriminating subunits 7OE, 8a* and 8b* associated with superior quality. A new glutenin subunit 7b*+8 was found in Japanese germplasm Eshimashinriki. |
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| Keywords: | Triticum aestivum L. Quality SDS-PAGE MALDI-TOF-MS HMW-GS |
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