葡萄双受精过程的形态学观察

观察了葡萄双受精过程中雌雄性核的融合。结果表明:花粉管通过珠孔穿入退化助细胞,释放出二精子;精子核接近并贴附在卵核和次生核上,二者核膜融合,精核沉入卵核及次生核,染色质松解,并与雌性核染色质融合,出现雄性核仁,两性核仁融合,形成合子及初生胚乳核。卵核完成受精较次生核晚。受精属有丝分裂前型。精核进入卵核前看到了它的核仁。少数胚囊具有两套卵器。

A Morphological Observation of the Process of Double Fertilization of Vitis. L.

We made an observation of the process of the fusion of male and fe- male nuclei in the double fertilization Vitis.L.The results indicated: after the pollen tube pieced into the embryo sac through the micropyle, two sperms were released.Sperm nucleus is close and presses close to the egg nucleus and the secondary nucleus.Thus,the fusion of nuclear mem- brance between the male and female nuclei occured.The sperm nucleus sinks into the egg nucleus and the secondary nucleus,and the male chromatin disperses and fuses with the female chromatin,from which the male nu- cleus arises.Thus far,the male and female nuclei are fused together to form the zygote and the primary endosperm nucleus.The completion of fertili- zation in the egg nucleus is later than that in the secondary nucleus.This fertilization belongs to the premitotic type.We also observed its nucleus before the sperm nucleus entered into the egg nucleus.A few of embryo sac contain two sets of egg apparatus.