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| 海芋根状茎的离体培养与植株再生 | |
| 引用本文： | 杨翠芹,刘运权,方颖,秦耀国,刘伟. 海芋根状茎的离体培养与植株再生[J]. 北方园艺, 2007, 0(5): 212-213 |
| 作者姓名： | 杨翠芹 刘运权 方颖 秦耀国 刘伟 |
| 作者单位： | 1. 四川农业大学农学院,雅安,625014;华南农业大学生命科学学院,广州,510642 2. 华南农业大学生命科学学院,广州,510642 3. 四川农业大学林学园艺学院,雅安,625014 |
| 摘 要： | 以海芋的根状茎为外植体,探索了不同的生长调节剂配比对愈伤组织诱导、增殖与分化的影响.结果表明:含TDZ 0.5 mg/L的MS培养基上愈伤诱导率最高,达到53.3%;4mg/LBA 0.1 mg/L NAA生长调节剂浓度有利于愈伤组织的增殖;附加1 mg/L BA 0.05 mg/LNAA的培养基上芽分化率最高;下表面切除0.1～0.2 cm有利于愈伤组织增殖与分化.不定芽在无生长调节剂的1/2MS培养基中都能生根. |
| 关 键 词： | 海芋 离体培养 愈伤增殖 分化 |
| 文章编号： | 1001-0009(2007)05-0212-02 |
| 收稿时间： | 2007-01-15 |
| 修稿时间： | 2007-01-15 |
In Vitro Culture and Plantlet Regeneration with Rhizome of Alocasia macrorrhiza |
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| YANG Cui-qin,LIU Yun-quan,FANG Ying,QIN Yao-guo,LIU Wei. In Vitro Culture and Plantlet Regeneration with Rhizome of Alocasia macrorrhiza[J]. Northern Horticulture, 2007, 0(5): 212-213 | |
| Authors: | YANG Cui-qin LIU Yun-quan FANG Ying QIN Yao-guo LIU Wei |
| Affiliation: | 1. College of Agrleulture,Siehuan Agricultural Unlversity,Yaan 625014,2. College of Life Selenee,South China Agricultural University,Guangzhou 510642;3. College of Forestry and Hortleulture,Sichuan Agricultural University,Yaan 625014 |
| Abstract: | Effects of different growth regulators combination on callus inducement,proliferation and differentiation were studied with rhizomes of Alocasia macrorrhiza as explants.Results showed that callus inducement rate was maximum and attained 53.3% in MS culture medium containing 0.5mg/L TDZ;It was advantageous for callus to proliferate with 4mg/L BA 0.1 mg/L NAA;Explants possessed the highest differentiation rate in culture medium with 1mg/L BA 0.05mg/L NAA.Callus excised 0.1~0.2cm thick in below surface was prone to proliferate and differentiat.When shoots were inserted into culture medium of 1/2MS,all of them produced roots. |
| Keywords: | Alocasia rnacrorrhiza In vitro culture Callus proliferation Differentiation |
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