| 奶牛乳房炎源金黄色葡萄球菌溶原性噬菌体的诱导及其裂解能力分析 |
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| 引用本文： | 常军帅,张琪,程露露,屈勇刚,梁晏,李娜,张小玉,李彦芳.奶牛乳房炎源金黄色葡萄球菌溶原性噬菌体的诱导及其裂解能力分析[J].中国奶牛,2022(2):27-31. |
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| 作者姓名： | 常军帅 张琪 程露露 屈勇刚 梁晏 李娜 张小玉 李彦芳 |
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| 作者单位： | 石河子大学动物科技学院;动物疾病防控兵团重点实验室 |
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| 基金项目： | 国家自然科学基金(32160847);动物疾病防控兵团重点实验室开放课题(2020BTDJ03);八师石河子市促进科技成果转化引导计划(2019ZH05);石河子大学成果转化与技术推广计划(CGZH201908)。 |
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| 摘 要： | 为研究奶牛乳房炎源金黄色葡萄球菌中溶原性噬菌体的存在情况及其裂解能力,对采集的45份临床型乳房炎乳样进行金黄色葡萄球菌分离及PCR鉴定,通过丝裂霉素C对分离出的金黄色葡萄球菌进行诱导溶原性噬菌体,对诱导出的噬菌体进行纯化并测定其裂解能力.结果表明分离到了16株金黄色葡萄球菌,分离率为35.56％(16/45);诱导出了...
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| 关 键 词： | 溶原性噬菌体 金黄色葡萄球菌 乳房炎 丝裂霉素C |
Induction and Lytic Ability Analysis of Lysogenic Phage of S. aureus from Dairy Cow Mastitis |
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| CHANG Jun-shuai,ZHANG Qi,CHENG Lu-lu,QU Yong-gang,LIANG Yan,LI Na,ZHANG Xiao-yu,LI Yan-fang.Induction and Lytic Ability Analysis of Lysogenic Phage of S. aureus from Dairy Cow Mastitis[J].China Dairy Cattle,2022(2):27-31. |
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| Authors: | CHANG Jun-shuai ZHANG Qi CHENG Lu-lu QU Yong-gang LIANG Yan LI Na ZHANG Xiao-yu LI Yan-fang |
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| Institution: | (College of Animal Science and Technology,Shihezi University,Shihezi 832003;Key Laboratory of Control and Prevention of Animal Disease,Xinjiang Production&Construction Corps,Shihezi 832003) |
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| Abstract: | To study the existence of lysogenic phages in S. aureus from dairy cow mastitis and their lytic ability.Isolation of S. aureus and PCR identification were performed on 45 samples of clinical mastitis milk. Mitomycin C was used to induce lysogenic phage of S. aureus, and the lysis of the induced phage was determined. Capacity and purification.The results showed that 16 strains of S. aureus were isolated, and the isolation rate was 35.56%(16/45).Five strains of lysogenic phages were induced, with an induction rate of 31.25%(5/16). The plaque is a round plaque with a transparent center and blurred edges.These 5 bacteriophages only lyse S. aureus, with the highest lysis rate being 58.82%(10/17) and the lowest being 11.76%(2/17). This study can provide a reference for the research on the occurrence of mastitis in dairy cows, and it can also provide certain help for the prevention and control of S. aureus which is the source of mastitis. |
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| Keywords: | Lysogenic phage S aureus Mastitis Mitomycin C |
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