用于农药残留快速检测的生物传感器灵敏度筛选试验(简报)

为了提高乙酰胆碱酯酶生物传感器的灵敏度，使其更有效地应用于有机磷农药的快速检测。以商品乙酰胆碱酯酶（家蝇）和自制的鸡肝酶、鸡脑酶、鲤鱼脑酶为研究对象，比较了这4种乙酰胆碱酯酶的活性；检测了不同浓度的氧乐果、敌百虫和敌敌畏对4种酶的酶活抑制率。结果表明，鸡脑乙酰胆碱酯酶活性抑制率最高，3种农药对其的抑制率分别为：敌敌畏100 μg/L的抑制率为54.1%，敌百虫100 μg/L的抑制率为50.56%，氧化乐果100 μg/L的抑制率为24.16%。鸡脑酶廉价易得，且易于纯化，与自制的鸡肝酶和鲤鱼脑酶相比，乙酰胆碱酯酶的活性抑制率显著较高。因此鸡脑酶可以作为农产品农药残留快速检测用酶。

Screening experiment of biosensor sensitivity in rapid detection of organophosphorous pesticide residues

In order to enhance the sensitivity of acetylcholinesterase biosensor and detect organophosphorous pesticides effectively and rapidly,four different sources of enzymes used for detecting pesticides were tested and compared.Homogenates from chicken liver,chicken brain and cyprinoids brain,respectively were home-made in laboratory and acetylcholinesterase(AChE,from Musca domestica)was purchased.The inhibition rates of AChE activities under different concentrations of omethoate,trichlorfon and dichlorvos were ...