| 玉米小斑病抗性种质鉴定及生理生化分析 |
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| 引用本文： | 高刚强,刘海芳,陈银银,燕树锋,刘天学,温涛,高意帆,赵霞,穆心愿,杨豫龙. 玉米小斑病抗性种质鉴定及生理生化分析[J]. 玉米科学, 2024, 32(3): 114-120 |
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| 作者姓名： | 高刚强 刘海芳 陈银银 燕树锋 刘天学 温涛 高意帆 赵霞 穆心愿 杨豫龙 |
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| 作者单位： | 河南农业大学农学院, 郑州 450046;河南农业职业学院园艺园林学院, 郑州 451450;河南省农业科学院粮食作物研究所/河南省玉米绿色精准生产国际联合实验室, 郑州 450002 |
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| 基金项目： | 国家自然科学基金(32061143033)、河南省科技攻关项目(232102110255)、河南省农业科学院自主创新项目(2024ZC015)、2024年河南省留学人员科研择优项目 |
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| 摘 要： | 以200份玉米优良自交系为材料，2022-2023年夏分别进行小斑病菌的田间接种，综合两年数据共鉴定出高抗小斑病的玉米自交系3份，分别为K22、CML50和齐319；筛选到包括Mo17在内的抗性玉米自交系24份。选取K22和B73两个具有不同抗性水平的玉米自交系进行小斑病菌的室内接种。结果表明，48 h和72 h时，自交系K22叶片的病斑数、病斑面积和病斑面积比等各项指标均显著优于自交系B73，进一步验证田间鉴定的准确性。48 h或72 h时，高抗小斑病自交系K22与感病对照B73相比，5种防御酶活性增加14.32%～70.45%。
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| 关 键 词： | 玉米 小斑病 种质资源 防御酶 真菌病害 抗病性 |
| 收稿时间： | 2023-12-06 |
Identification of Resistant Maize Germplasm for Southern Leaf Blight and Physiological Characteristics Measurement |
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| GAO Gang-qiang,LIU Hai-fang,CHEN Yin-yin,YAN Shu-feng,LIU Tian-xue,WEN Tao,GAO Yi-fan,ZHAO Xi,MU Xin-yuan,YANG Yu-long. Identification of Resistant Maize Germplasm for Southern Leaf Blight and Physiological Characteristics Measurement[J]. Journal of Maize Sciences, 2024, 32(3): 114-120 |
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| Authors: | GAO Gang-qiang LIU Hai-fang CHEN Yin-yin YAN Shu-feng LIU Tian-xue WEN Tao GAO Yi-fan ZHAO Xi MU Xin-yuan YANG Yu-long |
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| Affiliation: | College of Agronomy, Henan Agricultural University, Zhengzhou 450046;College of Horticulture and Landscape Architecture, Henan Vocational College of Agriculture, Zhengzhou 451450;Cereal Institute, Henan Academy of Agricultural Sciences/Henan International Joint Laboratory on Maize Precision Production, Zhengzhou 450002, China |
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| Abstract: | Two hundred excellent inbred lines of maize were used as materials, and the field inoculation of SLB pathogen were conducted during the summers of 2022 and 2023, respectively. Three highly resistant inbred lines, namely K22, CML50, and Qi319, were identified and 24 resistant inbred lines, including Mo17, were screened based on the two-year data. Additionally, two maize inbred lines with different levels of resistance, K22 and B73, were selected for indoor inoculations with the SLB pathogen and the results showed that the various indexes of leaf lesion number, leaf lesion area and leaf lesion area ratio for K22 were significantly better than those for B73 at 48 and 72 hours, which furtherly validated the accuracy of field identification. The activity increase of 5 defense enzymes ranged from 14.32 to 70.45% in the highly resistant inbred line K22 compared to the susceptible control B73 while 48 or 72 hours. |
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| Keywords: | Maize Southern leaf blight Germplasm resource Defensive enzyme Fungal disease Disease resistance |
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