玉米LRR-RLK基因家族鉴定和SIF亚家族表达模式分析

富含亮氨酸重复的类受体激酶(leucine-rich repeat receptor-like kinase，LRR-RLK)能够调节植物的生长发育、参与激素信号传导、抵御各种逆境胁迫，它是植物中已知最大的跨膜类受体蛋白激酶(receptor-like kinase,RLK)家族。SIF是LRR-RLK家族中的一个亚家族，该亚家族在拟南芥和棉花中被证明参与逆境胁迫。利用生物信息学方法对玉米B73基因组的LRR-RLK家族进行全基因组鉴定、系统进化、染色体定位和基因结构分析，基于LRR-RLK家族的分类结果，选择第Ⅰ亚家族SIF作为研究对象，分析该亚家族成员组织特异性表达以及对逆境胁迫的响应模式。结果表明，玉米LRR-RLK基因家族包含249个成员，这些LRR-RLK基因非均等地分布于玉米10条染色体上。系统进化分析可将其分为15类，各亚家族基因数量分布由1～51个不等。基于转录组数据的表达模式分析表明，玉米SIF亚家族基因在不同组织及逆境胁迫下的表达量不同，发现了多个可响应非生物胁迫(干旱胁迫、冷胁迫、热胁迫和盐胁迫)和生物胁迫(玉蜀黍平脐蠕孢、禾谷镰孢、瘤黑粉菌和蚜虫)的SIF基因。

Genome-Wide Identification of the LRR-RLK Gene Family and SIF Subfamily Gene Expression Profiling in Maize

The leucine-rich repeat receptor-like kinase(LRR-RLK) is one of the largest families of the receptor-like kinase(RLK) superfamily in plants which function in plant growth, development, abiotic and biotic stress responses. The Stress Induced Factor(SIF) subfamily is one of subclades in the LRR-RLK family. Four members of the SIF subfamily(AtSIF1-AtSIF4) in Arabidopsis and cotton have been reported to be involved in abiotic and biotic stress responses. Herein, we performed genome-wide identification and phylogenetic analysis of the LRR-RLK gene family in B73 genome in maize. Chromosome positions and gene structures of LRR-RLK family members were analyzed. There are 249 LRR-RLK members in the LRR-RLK family in maize. The LRR-RLK genes are distributed unevenly on 10 maize chromosomes. Phylogenetic analysis revealed 15 subfamilies in LRR-RLK gene family. The number of genes in each subfamily ranges from 1 to 51. Besides, we analyzed expression patterns of the SIF subfamily genes in different tissues and under different stresses. Several genes of the SIF subfamily responding to both abiotic stresses(drought stress, cold stress, heat stress or salt stress) and biotic stresses(Cochliobolus heterostrophus, Fusarium graminearum, Ustilago maydis or Rhopalosiphum maidis infections) were identified.