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花生栽培种(Arachis hypogaea)类型间遗传差异的SSR分析
引用本文:洪彦彬,梁炫强,陈小平,林坤耀,周桂元,李少雄,刘海燕. 花生栽培种(Arachis hypogaea)类型间遗传差异的SSR分析[J]. 分子植物育种, 2008, 6(1): 71-78
作者姓名:洪彦彬  梁炫强  陈小平  林坤耀  周桂元  李少雄  刘海燕
作者单位:广东省农业科学院作物研究所,广州,510640
基金项目:国家高技术研究发展计划(863计划) , 广东省自然科学基金 , 广东省农业科技攻关项目
摘    要:本文采用110对SSR引物分析28份花生栽培种资源(7份多粒型、5份龙生型、8份普通型和8份珍珠豆型)间的遗传差异,其中有46对引物在不同品种间检测出2~9个等位基因,多态性信息量(PIC)为0.080~0.869.对28份材料的聚类分析表明,SSR聚类结果与根据形态特征的分类结果基本一致,但在多粒型品种上存在一定差异.标记pPGSseq15C12在本研究中所有的珍珠豆型品种上均扩增出特异性条带,序列分析表明该标记在珍珠豆型与其它类型间扩增片段的差异是由于ATT重复次数不同所致.

关 键 词:花生  栽培种类型  遗传差异  SSR多态性  花生栽培种  hypogaea  类型  遗传差异  分析表  Peanut  Differences  Genetic  重复次数  扩增片段  序列  特异性条带  研究  存在  分类结果  形态特征  聚类  材料  信息量  多态性

Genetic Differences in Peanut Cultivated Types (Arachis hypogaea) Revealed by SSR Polymorphism
Hong Yanbin,Liang Xuanqiang,Chen Xiaoping,Lin Kunyao,Zhou Guiyuan,Li Shaoxiong,Liu Haiyan. Genetic Differences in Peanut Cultivated Types (Arachis hypogaea) Revealed by SSR Polymorphism[J]. Molecular Plant Breeding, 2008, 6(1): 71-78
Authors:Hong Yanbin  Liang Xuanqiang  Chen Xiaoping  Lin Kunyao  Zhou Guiyuan  Li Shaoxiong  Liu Haiyan
Affiliation:Hong Yanbin Liang Xuanqiang Chen Xiaoping Lin Kunyao Zhou Guiyuan Li Shaoxiong Liu Haiyan Crops Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,51064
Abstract:The 28 accessions of peanut germplasms (Arachis hypogaea L.) were employed to analyze their genetic differences in the cultivated types by using SSR-PCR approach. The tested germplasm would be assigned as four types including fastifgiata type (7 accessions), hirsute type (5 accessions), hypogaea type (8 accessions) and vulgaris type (8 accessions). Forty-six of 110 pairs of SSR primers might detect the polymorphism in the tested peanut germplasms with 2~9 alleles per locus. The value of PIC (polymorphism in...
Keywords:A rachis hypogaea L.   Cutivated types   Gnentic difference   SSR polymorphism
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