| 影响动物胚胎干细胞克隆与分化因素的研究进展 |
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| 引用本文： | 安立龙,高志敏,效梅,杨奇,窦忠英.影响动物胚胎干细胞克隆与分化因素的研究进展[J].西北农业学报,2001,10(2):106-109,118. |
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| 作者姓名： | 安立龙 高志敏 效梅 杨奇 窦忠英 |
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| 作者单位： | 西北农林科技大学畜牧兽医学院, |
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| 基金项目： | 国家自然科学基金资助(批准号:39670359),国家攻关课题资助(编号:96-C0103),校青年教师专项基金资助. |
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| 摘 要： | 系统介绍了在动物胚胎干细胞分离与克隆中使用的培养液和添加物的特性及培养液、添加物和遗传因素对动物胚胎干细胞克隆及分化的影响。在 DMEM培养基中添加优质血清、表皮生长因子、胰岛素生长因子、白血病生长因子等能显著提高胚胎干细胞的克隆率 ,生长因子通过促进细胞 DNA和蛋白质合成 ,抑制细胞程序性死亡来提高 ES细胞克隆率。叶酸、二甲基亚砜、神经生长因子、碱性成纤维细胞因子等可诱导动物胚胎干细胞在体外分化 ,形成神经细胞、造血细胞、心肌细胞和肌肉组织等。
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| 关 键 词： | 胚胎干细胞 分化 培养液 添加剂 生长因子 遗传基因 |
| 文章编号： | 1004-1039(2001)02-0106-04 |
| 收稿时间： | 2000/8/10 0:00:00 |
Progress in Research of Some Factors Influencing on Clone and Differentiation of Embryonic Stem Cell in Mammal Animal |
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| AN Li-long,GAO Zhi-min,XIAO Mei,YANG Qi and DOU Zhong-ying.Progress in Research of Some Factors Influencing on Clone and Differentiation of Embryonic Stem Cell in Mammal Animal[J].Acta Agriculturae Boreali-occidentalis Sinica,2001,10(2):106-109,118. |
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| Authors: | AN Li-long GAO Zhi-min XIAO Mei YANG Qi and DOU Zhong-ying |
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| Institution: | The key and open laboratory for Agriculture ministry of china, College of Animal Science and Veterinary Medicine, Nortywest Sci-Tech University of Agirculture and Forestry Yangling Shaanxi 712100, China;The key and open laboratory for Agriculture ministry of china, College of Animal Science and Veterinary Medicine, Nortywest Sci-Tech University of Agirculture and Forestry Yangling Shaanxi 712100, China;The key and open laboratory for Agriculture ministry of china, College of Animal Science and Veterinary Medicine, Nortywest Sci-Tech University of Agirculture and Forestry Yangling Shaanxi 712100, China;The key and open laboratory for Agriculture ministry of china, College of Animal Science and Veterinary Medicine, Nortywest Sci-Tech University of Agirculture and Forestry Yangling Shaanxi 712100, China;The key and open laboratory for Agriculture ministry of china, College of Animal Science and Veterinary Medicine, Nortywest Sci-Tech University of Agirculture and Forestry Yangling Shaanxi 712100, China |
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| Abstract: | The characteristics of culture medium and additive and the influence of culture medium,additive and genetic factors on isolafion and clone of embryonic stem cell were introduced.The ration of clone of embryonic stem cell were improved in DMEM culture medium supplemented with new bovine serum,LIF,EGF,IGF.The neuronal,hematopoietic,smooth muscle,cardiac muscle,adipose cell derived from embry onic stem cell were produced by Retinoic acid,DMSO,db-cAMP,brain derived neurotrophic factor,and clone of embryonic stem cell in mammal animal were discussed in this paper. |
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| Keywords: | Embryonic stem cell Differentiation Culture medium Additive Growth factor Geneticfactor |
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