沈阳市及周边地区规模化猪场猪繁殖与呼吸障碍综合征疫苗免疫效果及病原调查

为了解沈阳及周边地区猪繁殖与呼吸障碍综合征病毒感染情况和疫苗免疫效果，应用已建立的猪繁殖与呼吸障碍综合征病毒RT—PCR．检测方法在2011年1月～2013年6月期间，对来自辽宁省沈阳、大连、鞍山等16个市县地区的猪场和屠宰场的送检样品进行检测。结果发现，2011年辽宁省猪繁殖与呼吸障碍综合征病毒的阳性率为9．28％，2012年为27．3％，2013年上半年为0．6％，3年平均阳性率为12．1％；同时选择沈阳市5家规模化养猪场3年采集血清902份进行猪繁殖与呼吸障碍综合征疫苗免疫效果的评价，结果显示，2011年5个猪场猪繁殖与呼吸障碍综合征疫苗免疫抗体合格率为97．3％，2012年为98．8％，2013年上半年为94．8％，3年平均阳性率为97．5％；另外，对沈阳市某猪场免疫母猪所产仔猪的母源抗体进行为期7周的监测，发现，仔猪猪繁殖与呼吸障碍综合征病毒母源抗体在7日龄时达到最大值，直到21日龄维持在较高水平，之后呈现下降趋势。

Investigation of the Protective effect of Porcine reproductive and respiration syndrome vaccine and Pathogen on Large scale pig farms in Shenyang

In order to comprehend the Protective effect of Porcine reproductive （PRRS） and respi- ration syndrome vaccine and its Pathogen on Large scale pig farms of Shen-yang city and surround- ing area, RT-PCR method was used to detect samples from sixteen towns in Shenyang, Dalian, Anshan and ect. The results showed that PRRS positive rate is 9.28% in 2011, 27.3% in 2012, 0.6% in the first six months of 2013 and the average positive rate is 12.1%. ELISA was used to detect the Pro- tective effect of the PRRS vaccine, 902 serum samples was tested from 5 Large scale pig farms in Shenyang, the results showed that Antibody qualification rate is 97.3% in 2011, 98.8% in 2012, 94.8% in 2013, Average qualification rate is 97.5%. The maternal antibody was monitored for 7 weeks in a pig farm, The results showed that the maternal antibody of PRRS was reached the maximum value on the seventh day, maintaining at the higher level until the 2Ith day, and then showed a de- clined trend.