| 饲料和鸡肉中利巴韦林的测定--超高效液相色谱-串联质谱法 |
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| 引用本文： | 刘凯,;王丽娜,;李建忠.饲料和鸡肉中利巴韦林的测定--超高效液相色谱-串联质谱法[J].现代畜牧兽医,2014(5):15-19. |
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| 作者姓名： | 刘凯 ;王丽娜 ;李建忠 |
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| 作者单位： | [1]辽宁省兽药饲料畜产品质量安全检测中心,辽宁沈阳110016; [2]安捷伦科技中国有限公司,北京100102 |
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| 摘 要： | 本研究建立了饲料和鸡肉中利巴韦林的UPLC-MS/MS分析方法。样品经三氯乙酸-乙腈溶液提取，离心后提取液经PBA固相萃取小柱净化，0.1 mol/L甲酸水溶液溶解，进行UPLC-MS/MS分析。结果显示，利巴韦林在10.0～500.0 g/L浓度范围内线性关系良好，r2=0.9991。以浓缩饲料、配合饲料、预混料和鸡肉等为添加基质，其回收率在90.0%～114.5%，检出限为25.0 g/kg，定量限为50.0 g/kg。表明本方法灵敏度高、专属性好、操作简单、结果可靠，可满足饲料和鸡肉中利巴韦林的分析检测。
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| 关 键 词： | 利巴韦林 饲料 鸡肉 |
Determination of Ribavirin in Feeds and chicken by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| Institution: | Liu Kai, Wang Lina, Li Jianzhong (1. Animal feed quality and safety of veterinary testing centre in Liaoning province, Liaoning Shenyang 110016; 2. Agilent Technologies Co.,Ltd., Beijing 100102) |
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| Abstract: | A method of Ultra Performance Liquid Chromatography-Tandem mass spectrometry（UPLC-MS/MS） for determination of ribavirin in feeds and chicken was developed. The drugs were extracted with acetonitrile/trichloroacetic acid from analyte, then cleaned up with PBA cartridges. The elu-ent was assayed by UPLC-MS/MS. The detection and quantification limits were 25.0 μg/kg and 50.0 μg/kg respectively. The average recoveries were 90.0%~114.5%. The real sample tests showed this meth-od can be used for the sensitive and accurate determination of ribavirin in feeds and chicken. |
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| Keywords: | UPLC-MS/MS Ribavirin Feeds Chicken UPLC/MS/MS |
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