| 黄瓜叶片DNA的提取及AFLP分析体系的建立 |
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| 引用本文： | 孙涌栋,张兴国,陈碧华,李贞霞,李新峥.黄瓜叶片DNA的提取及AFLP分析体系的建立[J].西南农业学报,2007,20(4):857-859. |
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| 作者姓名： | 孙涌栋 张兴国 陈碧华 李贞霞 李新峥 |
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| 作者单位： | 1. 河南科技学院园林学院,河南,新乡,453003
2. 西南大学园艺园林学院,重庆,400715 |
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| 基金项目： | 教育部优秀青年教师资助计划项目[教育司（2003）355号];河南科技学院重点科研项目资助基金（6011） |
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| 摘 要： | 以黄瓜叶片为研究材料提取基因组DNA并进行了AFLP扩增。对影响AFLP分析体系的关键因素进行了比较和优化,探索出适宜黄瓜的DNA提取方法,并建立了AFLP分析体系,得到了较为清晰可辨的AFLP指纹图谱,为进行黄瓜的遗传多样性分析、遗传图谱构建和基因定位等研究奠定基础。
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| 关 键 词： | 黄瓜 cDNA-AFLP 分析体系 |
| 文章编号： | 1001-4829（2007）04-0857-03 |
| 修稿时间： | 2006-12-10 |
Extraction of DNA and establishment of AFLP analysis system for cucumber leaves |
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| SUN Yong-dong,ZHANG Xing-guo,CHEN Bi-hua,LI Zhen-xia,LI Xin-zheng.Extraction of DNA and establishment of AFLP analysis system for cucumber leaves[J].Southwest China Journal of Agricultural Sciences,2007,20(4):857-859. |
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| Authors: | SUN Yong-dong ZHANG Xing-guo CHEN Bi-hua LI Zhen-xia LI Xin-zheng |
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| Institution: | 1. College of landscape, Henan Institute of Science and Technology, Henan Xinxiang 453003, China ;2. College of Horticulture and I.and- scape, Southwest University,Chongqing 400715 ,China |
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| Abstract: | Genomic DNA was extracted from cucumber leaves and AFLP was carried out with the DNA.The important factors affecting the AFLP analysis system were compared and optimized.A better procedure for DNA extraction and AFLP analysis of cucumber was suggested.And a distinct electrophoretogram from the AFLP amplification could be showed.By means of AFLP analysis system,genetic diversity,map construction and gene location of cucumber could be studied. |
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| Keywords: | cucumber DNA extraction AFLP |
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