菊苣种质资源遗传多样性的SRAP研究

利用SRAP分子标记技术对来自5大地理类群的48份菊苣材料的遗传多样性进行了研究。用筛选的28对引物对48份材料的DNA进行PCR扩增,获得以下结论:1)28个位点共检测到333个等位基因,平均为11.89个,多态性位点率(P)平均为95.09%;多态性信息含量(PIC)范围为0.23(me3+em3)～0.44(me1+em10),平均为0.33;2)材料间遗传相似系数(GS)范围在0.55～0.89,平均GS值为0.69;地理类群间的GS值在0.63～0.96,其中荷兰(P,95.52%)和意大利(P,95.38%)类群遗传多样性丰富,表明供试菊苣种质具有丰富的遗传变异;3)根据研究结果进行聚类分析和主成分分析,可将48份菊苣材料分为五大类,来自相同地理类群的材料基本能聚为一类,呈现出一定的地域性分布规律。

Genetic diversity of Cichorium intybus germplasm resources using sequence-related amplified polymorphism (SRAP) molecular markers

Genetic diversity of 48 accessions of all Cichorium intybus collected from 5 geographical groups was analyzed using sequence-related amplified polymorphism (SRAP) molecular markers with 28 SRAP primer pairs. 1) A total of 333 alleles were detected at 28 loci, an average of 11.89. Occurrence of polymorphic sites (P) was 95.09% and the polymorphic information content (PIC) ranged from 0.23(me3+em3)to 0.44(me1+em10), an average of 0.33. 2) The genetic similarity (GS) among all accessions ranged from 0.55 to 0.89 with an average of 0.69. For all geographical groups, the GS value ranged from 0.63 to 0.96. Among these groups, a high genetic diversity was observed in groups from the Netherlands (P, 95.52%) and Italy (P, 95.38%). These results suggested that there was a high genetic diversity among all C. intybus accessions tested. 3) Based on cluster and principal component analyses, 48 accessions could be divided into five groups by the nearest phylogenetic relationship, and the five groups reflected the five geographical origins.