| 稻瘟病菌胁迫下水稻miRNA的表达 |
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| 引用本文： | 姜兆远,任金平,刘晓梅,夏海丰,洪德志,高玮,袁媛.稻瘟病菌胁迫下水稻miRNA的表达[J].安徽农业科学,2013(17):7410-7412,7417. |
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| 作者姓名： | 姜兆远 任金平 刘晓梅 夏海丰 洪德志 高玮 袁媛 |
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| 作者单位： | 中国农业科技东北创新中心,吉林长春130033;吉林省农业科学院植保所,吉林公主岭136100;吉林省通化市农业科学院,吉林梅河口,135007;吉林省农业技术推广总站,吉林长春,130021 |
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| 摘 要： | 目的]分析水稻抗病初期miRNA及靶基因的表达情况。方法]运用Affymetrix基因表达谱芯片及miRNA芯片对接种稻瘟菌的水稻进行关联分析,通过分子注释系统平台(MAS 3.0)对差异表达miRNA靶基因的功能进行注释分析。结果]研究显示表达差异以Fold change绝对值大于1.5倍进行筛选,共筛选到41个miRNA,与靶基因存在调控关系的有25个。结论]水稻抗病初期存在表达差异的miRNA,一些靶基因在水稻抗病反应中起关键作用。
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| 关 键 词： | 稻瘟病 miRNA 基因芯片 |
The Expression of Rice MicroRNA Response to Blast Fungus Stress |
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| Institution: | JIANG Zhao-yuan;Northeast Agricultural Research Center of China; |
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| Abstract: | Abstract Objective ] To investigate the responsive functions of rice miRNAs and mRNA under blast fungus stress Method ] miRNA and mR- NA expression in blast fungus-stressed rice were profiled using two microarray assay. Go （Gene Ontology） analyses were conducted on differenti- ally expressed genes using a free web-based Molecular Annotation System 3.0 （MAS 3.0）. Result ] A total of 41 blast-responsive miRNA at a one point five-fold filter were identified. In addition, the mRNA levels of some targets were negatively correlated with the corresponding the 25 miRNAs under rice blast stress. |
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| Keywords: | Rice blast miRNA Genechip |
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