利用SSR分子标记分析彩色小麦的亲缘关系与遗传多样性

为探讨彩色小麦的亲缘关系和遗传多样性, 利用305对多态性SSR引物检测了18个彩色小麦品种(系)。共检测到1 084个等位变异, 平均每对引物检测到3.6个。红粒、紫红粒小麦的等位变异数在7个部分同源群的排序为HG3>HG2>HG1>HG4>HG5>HG7>HG6, 黑粒和蓝粒小麦的排序为HG3>HG2>HG1和HG4>HG5>HG7>HG6。不同粒色小麦的SSR标记多态信息含量相近, 其中红粒和紫红粒小麦为0.525~0.543, 黑粒和蓝粒小麦为0.517~0.545。18个品种(系)的遗传差异极显著(P=0.0001), 遗传相似系数在0.41~0.76之间。UPGMA聚类分析将18份材料分为6群,类群分布与亲缘关系及其地域相关。

Relationships and Genetic Diversity of Colored-Grain Wheat Detected by SSR Markers

To reveal the relationship and genetic diversity of colored-grain wheat,we analyzed 18 varieties(lines) with red,purple,blue,and black seed coats using 305 polymorphic SSR markers.A total of 1084 alleles were detected with 3.6 alleles per locus.The allele numbers in seven homoeologous groups(HG) were aligned as HG3>HG2>HG1>HG4>HG5>HG7>HG6 in red and purple wheat and HG3>HG2>HG1 and HG 4>HG5>HG7>HG6 in black and blue wheat.The polymorphic information content(PIC) values in the seven homoeologous groups were similar,which were 0.525-0.543 in red and purple wheat and 0.517-0.545 in black and blue wheat.Among the 18 colored-grain varieties(lines),genetic difference was significant(P = 0.0001),and the genetic similarity coefficients ranged from 0.41 to 0.76.According to UPGMA analysis,the 18 varieties(lines) were classified into six groups,which were in relation to variety kindred and geographic distance.