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| 转基因水稻中抗虫基因的遗传分析 | |
| 引用本文： | 李永春,孟凡荣,薛庆中.转基因水稻中抗虫基因的遗传分析[J].中国农学通报,2005,21(1):26-26. |
| 作者姓名： | 李永春 孟凡荣 薛庆中 |
| 作者单位： | 1. 山西农业大学农学院,太谷,030801 2. 中国农业大学作物遗传育种系,北京,100094 3. 浙江大学农学系,杭州,310029 |
| 基金项目： | 国家自然科学基金(39870421). |
| 摘 要： | 通过PCR检测及正、反交试验对转双价抗虫基因水稻材料的8个T1代株系中抗虫基因的遗传特性进行了分析，结果表明：6个株系中抗虫基因的分离符合3:1的孟德尔单基因分离规律；1个株系中阳性和阴性植株之比接近于15:1，该株系中抗虫基因可能以双拷贝插入到基因组中的两个非连锁位点；1个株系中阳性和阴性植株之比极显著偏离3:1(接近于1:1)，进一步分析表明该偏分离现象可能与转基因花粉竞争能力下降有关。 |
| 关 键 词： | 水稻 抗虫基因 遗传分析 |
Hereditary Analysis of Insecticidal Transgenes in Transgenic Rice Plants |
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| Li Yongchun,Meng Fanrong,Xue Qingzhong.Hereditary Analysis of Insecticidal Transgenes in Transgenic Rice Plants[J].Chinese Agricultural Science Bulletin,2005,21(1):26-26. | |
| Authors: | Li Yongchun Meng Fanrong Xue Qingzhong |
| Institution: | (1College of Agronomy, Shanxi Agricultural University, Taigu 030801; 2Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100094; 3Agronomy Department, Zhejiang University, Hangzhou, 310029) |
| Abstract: | The hereditary characteristics of 8 Ti lines harboring two insecticidal genes were studied via PCR analysis and cross test, the results showed that the insecticidal genes transmitted as Mendel rule of 3:1 in 6 lines. In one line, the ratio of positive plants for insecticidal gene to negative ones was approximately 15:1, indicating that the insecticidal genes might be incorporated in the two unlinked loci in the genome. In another line, the ratio was significantly deviated from the ratio 3:1 (approximately 1:1) and the further study showed that the phenomena might be related to decreased competing ability of transgenic pollens. |
| Keywords: | Oryza sativa Insecticidal genes Heredity analysis |
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