| 马铃薯茎尖脱毒培养方法优化研究 |
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| 引用本文： | 齐恩芳,王一航,张武,李玉萍. 马铃薯茎尖脱毒培养方法优化研究[J]. 中国马铃薯, 2007, 21(4): 200-203 |
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| 作者姓名： | 齐恩芳 王一航 张武 李玉萍 |
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| 作者单位： | 甘肃省农业科学院马铃薯研究所,兰州,730070 |
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| 基金项目： | 国家发展和改革委员会资助项目 |
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| 摘 要： | 以甘肃省主栽品种陇薯6号为材料,研究了茎尖大小及培养基中激素和活性炭对马铃薯茎尖培养成活的影响。结果表明,叶原基数为2的茎尖脱毒效果最好;MS培养基中加入0.5 mg·L~(-1)6-BA、0.1mg·L~(-1)GA_3和0.1 mg·L~(-1) NAA有助于陇薯6号茎尖分生组织分化;加入0.05%活性炭,茎尖成苗时间提前了13 d,成苗率增加了7.5%。
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| 关 键 词： | 马铃薯 茎尖培养 成苗率 |
| 文章编号： | 1672-3635（2007）04-0200-04 |
| 修稿时间： | 2007-04-10 |
Optimization of Meristem Culture for Elimination of Viruses in Potato |
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| Qi Enfang,Wang Yihang,Zhang Wu,Li Yuping. Optimization of Meristem Culture for Elimination of Viruses in Potato[J]. Chinese Potato, 2007, 21(4): 200-203 |
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| Authors: | Qi Enfang Wang Yihang Zhang Wu Li Yuping |
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| Affiliation: | Potato Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070, China |
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| Abstract: | Potato cultivar,Longshu No.6,was used in this experiment to study the effects on survival rate of meristem size,hormones and activated charcoal in miristem culture.The meristem with 2 primodial was optimal explant in vitro.Supplementation of 6-BA 0.5 mg·L~(-1),GA_3 0.1mg·L~(-1) or NAA 0.1mg·L~(-1) to MS promoted the differentiation of Longshu No.6 meristem tip.By supplementation of 0.05% activated charcoal to MS,the time of plant formation was advanced by 13 days and sprouting percentage was increased by 7.5%. |
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| Keywords: | potato meristem tip culture sprouting percentage |
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