湿地松猝倒病拮抗细菌的筛选与定殖

通过平板对峙培养,从320株分离于湿地松根部的拮抗细菌中筛选出27株对湿地松猝倒病病原菌有拮抗作用的菌株,再结合抑菌指数测定,进一步筛选出了抑菌作用最强的10株拮抗细菌.经16S rDNA分子测序方法鉴定,B4、C4、C23、C25、C28、C33、C41为洋葱伯克霍尔德菌,C3和C8为枯草芽孢杆菌,C27无法测定全部...

Screening and Colonization of Antagonistic Bacteria Against Damping-off of Slash Pine Seedlings

Twenty seven strains of bacteria with obviously antagonistic ability to pathogens of damping-off were screened out from 320 strains isolated from the soil near the roots of slash pine by the dual culture method.Then ten strains with strong antagonistic ability were selected out in accordance with the determination result of inhibition index.They were identified by 16S rDNA sequencing.Results show that seven isolates of B4,C4,C23,C25,C28,C33 and C41 are Burkholderia cepacia,two isolates of C3 and C8 are Bacillus subtilis,and C27 is an unknown species.B4 and C41 showed prominent control effects in the indoor experiment,while C23 showed a prominent control effect in the outdoor experiment.Ten strains could be colonized in the roots of slash pine,but the distribution of the ten strains was irregular in different parts of the root system.In the process of colonization,the quantity of the ten strains kept rising over time.At a certain hour,the amount of the ten antagonistic strains would reach a peak,then gradually reduced.