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| 银杏小孢子叶球蛋白提取和双向电泳体系优化(Ⅱ) | |
| 引用本文： | 杨妮娜,崔彬彬,孙宇涵,李允菲,王亚茹,陈欣,李云,邓高松,苗少波. 银杏小孢子叶球蛋白提取和双向电泳体系优化(Ⅱ)[J]. 东北林业大学学报, 2011, 39(8): 55-57 |
| 作者姓名： | 杨妮娜 崔彬彬 孙宇涵 李允菲 王亚茹 陈欣 李云 邓高松 苗少波 |
| 作者单位： | 1. 北京林业大学,北京,100083 2. 保定学院 3. 北京林业大学 4. 北京林业大学实验林场 |
| 基金项目： | 国家自然科学基金，林业行业专项 |
| 摘 要： | 以银杏小孢子叶球为材料,比较了不同的提取的方法、蛋白质裂解液组成、pH值范围以及不同质量浓度SDS-PAGE凝胶对双向电泳的影响.结果表明:TCA/丙酮/酚法比TCA/丙酮法更适合于银杏小孢子叶球蛋白的提取；含有硫脲的蛋白质裂解液Ⅱ,可显著提高蛋白质的溶解性,在2-DE图谱上可分辨出748个蛋白质点,比用蛋白质裂解液Ⅰ... |
| 关 键 词： | 银杏 TCA/丙酮/酚 双向电泳技术 |
Protein Extraction from Ginkgo biloba Starninate Strobilus and Optimization of Two-Dimensional Electrophoresis Protocol |
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| Yang Nina,Cui Binbin,Sun Yuhan,Li Yunfei,Wang Yaru,Chen Xin,Li Yun,Deng Gaosong,Miao Shaobo. Protein Extraction from Ginkgo biloba Starninate Strobilus and Optimization of Two-Dimensional Electrophoresis Protocol[J]. Journal of Northeast Forestry University, 2011, 39(8): 55-57 | |
| Authors: | Yang Nina Cui Binbin Sun Yuhan Li Yunfei Wang Yaru Chen Xin Li Yun Deng Gaosong Miao Shaobo |
| Affiliation: | (Teaching and Experimental Forestry Center of Beijing Forestry University) |
| Abstract: | The effects of different protein extraction methods,lysis buffers,pH ranges of IPG strip and different gel concentrations on two-dimensional gel electrophoresis were studied by using male flower buds of Ginkgo biloba as experimental materials.Result shows that,compared TCA-acetone-phenol protocol with TCA-acetone protocol,the former is more suitable.The protein solubility of the samples dissolved in lysis bufferⅡcontaining thiourea was greatly enhanced,and about 748 protein spots were detected on 2-DE gels,which was 620 more than that in lysis buffer I.More spots were assembled from pH 4 to pH 8.Replaced pH 3-10 strips with pH 4-7 strips,the spots were shown in a clearer way.When the proteins were extracted by the TCA-acetone-phenol method,dissolved with the lysis buffer II,with 17cm,pH 4-7 IPG strip,and 12% concentrations of the SDS-PAGE gel,they were better separated. |
| Keywords: | Ginkgo biloba TCA-acetone-phenol Two-dimensional gel electrophoresis |
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