| 抗朊蛋白单克隆抗体的免疫学特性研究 |
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| 引用本文： | 吴晓东,张永强,刘雨田,邹艳丽,刘珊,李林,王志亮. 抗朊蛋白单克隆抗体的免疫学特性研究[J]. 扬州大学学报(农业与生命科学版), 2009, 30(4) |
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| 作者姓名： | 吴晓东 张永强 刘雨田 邹艳丽 刘珊 李林 王志亮 |
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| 作者单位： | 1. 扬州大学农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;中国动物卫生与流行病学中心-国家外来动物疫病诊断中心-国家牛海绵状脑病参考实验室,山东,青岛,266032
2. 中国动物卫生与流行病学中心-国家外来动物疫病诊断中心-国家牛海绵状脑病参考实验室,山东,青岛,266032 |
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| 基金项目： | 国家"十一五"科技支撑计划项目，转基因生物新品种培育科技重大专项 |
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| 摘 要： | 以牛、羊重组朊蛋白(PrP)、正常牛、羊的脑组织匀浆、牛海绵状脑病(BSE)及羊痒病阳性的脑组织匀浆及切片为抗原,测定9株抗朊蛋白单克隆抗体的ELISA、免疫印迹及免疫组化等免疫学反应特性.结果表明:5株单抗可用于BSE及羊痒病的ELISA、免疫印迹检测;3株单抗可用于BSE及羊痒病的免疫组化检测;1株单抗在免疫印迹试验中只与羊痒病PrP~(Sc)的核心片段反应,结合该株单抗所识别的抗原表位PrP第78～93氨基酸(aa),证实BSE与羊痒病PrP~(Sc)的蛋白酶K消化位点存在差异.
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| 关 键 词： | 传染性海绵状脑病 朊蛋白 单克隆抗体 免疫学特性 |
Immunological characteristics of Anti-PrP McAbs |
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| WU Xiao-dong,ZHANG Yong-qiang,LIU Yu-tian,ZOU Yan-li,LIU Shan,LI Lin,WANG Zhi-liang. Immunological characteristics of Anti-PrP McAbs[J]. Journal of Yangzhou University(Agricultural and Life Science Edition), 2009, 30(4) |
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| Authors: | WU Xiao-dong ZHANG Yong-qiang LIU Yu-tian ZOU Yan-li LIU Shan LI Lin WANG Zhi-liang |
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| Affiliation: | WU Xiao-dong1,2,ZHANG Yong-qiang2,LIU Yu-tian2,ZOU Yan-li2,LIU Shan2,LI Lin2,WANG Zhi-liang2(1.Key Lab of Anim Inf Dis of MOA,Yangzhou Univ,Yangzhou 225009,China,2.Nat BSE Ref Lab,Nat Diag Cen for Exotic Ani Dis,CAH and EC,Qingdao 266032,China) |
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| Abstract: | In recent years, a series of anti-PrP monoclonal antibodies(McAbs) had been developed in our lab. In this research, 9 McAbs immuno-reactivities with PrP were tested in EIASA, Western blotting and immunohistoehemistry (IHC) assay. The results indicated that 5 McAbs could be used for the detection of BSE and Scrapie in Western blotting and EIASA, and 3 McAbs could be used for the detection of BSE and Scrapie in IHC assay. 1 McAb only recognized the core part of PrP~(Sc) from sheep in Western blotting, and its epitope was located in 78-93aa of PrP. Therefore, it could differentiate the proteinase K cleavage site of PrP~(Sc) from BSE and Scrapie. |
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| Keywords: | TSEs Prion Protein(PrP) monoclonal antibody(McAb) immunological characteristics |
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