| 鸡新城疫病毒抗原超滤技术研究 |
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| 引用本文： | 黄兵,王川庆,王莉莉. 鸡新城疫病毒抗原超滤技术研究[J]. 中国预防兽医学报, 2000, 22(2): 108-110 |
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| 作者姓名： | 黄兵 王川庆 王莉莉 |
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| 作者单位： | [1]南京农业大学动物医学院 [2]山东省农科院家禽研究所 |
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| 基金项目： | 国家攀登计划项目!(85_41_0 2_0 6 ) |
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| 摘 要： | 应用分子筛过滤技术超滤浓缩鸡新城疫病毒，并就ＮＤＶ抗原量对鸡免疫应答的和了动态研究，试验结果显示，将ＮＤＶ对外开放尿囊毒浓缩４倍体积，ＨＡ效价提高了２个滴度，病毒回收率达１００％；病毒活性亦未发生改变。免疫１１天后，ＮＤ浓缩苗和ＮＤ－ＩＢＤ二联苗组鸡的ＮＤ－ＨＩ抗体水平远远高于免疫对照组，差异极显著。结果表明，所建超滤技术适用于ＮＤＶ怕的浓缩应用；适量增另ＮＤＶ怕量能够加快鸡的体液免疫应答，增强鸡
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| 关 键 词： | 鸡新城疫病毒 超滤 抗原量 免疫应答 |
| 修稿时间： | 1999－07－12 |
Ultra-filtering Technique for Newcastle Disease Virus Antigen |
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| HUANG Bing ,WANG Chuanqing ,WANG Lili ,MU Jianqing ,LI Feng ,CHEN Puyan. Ultra-filtering Technique for Newcastle Disease Virus Antigen[J]. Chinese Journal of Preventive Veterinary Medicine, 2000, 22(2): 108-110 |
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| Authors: | HUANG Bing WANG Chuanqing WANG Lili MU Jianqing LI Feng CHEN Puyan |
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| Affiliation: | HUANG Bing 1,WANG Chuanqing 1,WANG Lili 2,MU Jianqing 2,LI Feng 2,CHEN Puyan 1 |
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| Abstract: | The efficacy of ultra_filtering technique for Newcastle Disease virus and the effects of concentration of NDV antigen on chickens immune response were determined.The results showed that HA titer of ultra_filtered virus fluid increased 2 log2 when the virus fluid was concentrated by 3_fold,virus recovery ratio approached almost to 100% by HA titer.ND_HI titers were higher dramatically in chickens inoculated with concentrated ND and ND_IBD oil emulsion vaccine than nonconcentrated ND vaccine.These data indicated that the ultra_filtering technique is useful for concentration of NDV antigen and the ND vaccine containing adequate NDV antigen could prompt early specific humoral immune response in chickens. |
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| Keywords: | NDV Ultra_filtering technique Concentration of antigen Immune response |
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