绿盲蝽危害对棉花防御性酶活性及丙二醛含量的诱导

以2种转基因棉品种国抗22、sGK321及其亲本泗棉3号、石远321为材料,研究受绿盲蝽危害胁迫后,其叶片防御性酶活性及丙二醛含量等生理指标的动态。结果表明:接种绿盲蝽前后,2种转基因棉花品种叶片防御性酶活性及丙二醛含量与其亲本棉花相比差异均不显著,说明外源基因的导入对棉花各项生理指标的变化无影响。同一棉花品种在绿盲蝽不同刺吸诱导的时间中,叶片防御性酶活性及丙二醛含量差异均达到极显著水平;在品种和接虫时间互作效应中,SOD、MDA、PAL活性或含量差异达显著或极显著水平,说明品种与接虫时间在这几个指标水平上存在真实的交互作用,而其余生理指标的差异不显著。

Herbivore Stress by Lygus Isucorum Inducing Protective Enzyme Activity and MDA Content on Different Cotton Varieties

The dynamics of the activities of protective enzymes and MDA content in two kinds of genetically modified (GM) cotton varieties and their non-GM parents after being piercing-sucked by Lygus lucorum were studied. The results showed that: before and after the vaccination of Lygus lucorum, there were no significant difference on the activities of protective enzymes and MDA content between two kinds of GM cotton varieties and their non-GM parents, so it has no effect on physiological indices when exogenous genes were introduced into cotton. Throughout the period of Lygus lucorum's attacking, there was very significant difference in the activities of protective enzymes and MDA content to the same cotton variety; the three indicators, SOD, MDA and PAL activities or content, showed significant or very significant differences in the interaction effect between variety and bug accepting time, and the rest of the interaction was not significant.