| 云南39个野生蔷薇种间遗传多样性的SSR分析 |
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| 引用本文： | 许凤,李凌,邱显钦,唐开学,蹇洪英,李树发,王其刚,张颢.云南39个野生蔷薇种间遗传多样性的SSR分析[J].西南大学学报,2009,31(6). |
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| 作者姓名： | 许凤 李凌 邱显钦 唐开学 蹇洪英 李树发 王其刚 张颢 |
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| 作者单位： | 许凤,XU Feng(西南大学,园艺园林学院,重庆,400716;云南省农科院花卉研究所,昆明,650205);李凌,LI Ling(西南大学,园艺园林学院,重庆,400716);邱显钦,唐开学,蹇洪英,李树发,王其刚,张颢,QIU Xian-qin,TANG Kai-xue,JIAN Hong-ying,LI Shu-fa,WANG Qi-gang,ZHANG Hao(云南省农科院花卉研究所,昆明,650205) |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划)，云南省科技计划项目，国家林业科技支撑计划子课题 |
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| 摘 要： | 利用简单重复序列SSR(Simple Sequence Repeat)标记技术对蔷薇属39份野生材料的遗传多样性进行了研究.用筛选出的20对SSR引物对39份材料DNA进行PCR扩增,在20个位点上共检测到249个等位基因,每一位点的等位基因变幅为6～19个,平均12.5个.材料间遗传相似系数变化范围为0.142～0.800,表明39个云南野生蔷薇种间具有丰富的遗传多样性.本研究发现,在相似系数为0.34时,基于SSR标记进行UPGMA的聚类分析将39份蔷薇野生种分为10个组,与植物形态学分类结果大体一致.Abstract：The genetic diversity of 39 Rosa wild species was studied by SSR (Simple Sequence Repeat).Twenty pairs of primers were used in PCR amplification with genomic DNA as template. A total of 249 alleles were detected at 20 loci. The number of alleles per locus ranged from 6 to 19, with an average of 12.5. The genetic similar coefficient ranged from 0. 142 to 0. 800, which showed that great genetic diversity existed among the 39 Rosa wild species in Yunnan based on SSR molecular markers. UPGMA cluster analysis distinctly classified the 39 wild Rosa species into ten groups at the similar coefficient 0.34, which is consistent with botanical morphological classification.
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| 关 键 词： | 蔷薇属 分类 遗传多样性 |
Analysis of Genetic Diversity of Some Rosa Wild Species in Yunnan Based on SSR Markers |
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| XU Feng,LI Ling,QIU Xian-qin,TANG Kai-xue,JIAN Hong-ying,LI Shu-fa,WANG Qi-gang,ZHANG Hao.Analysis of Genetic Diversity of Some Rosa Wild Species in Yunnan Based on SSR Markers[J].Journal of Southwest Agricultural University,2009,31(6). |
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| Authors: | XU Feng LI Ling QIU Xian-qin TANG Kai-xue JIAN Hong-ying LI Shu-fa WANG Qi-gang ZHANG Hao |
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| Abstract: | |
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| Keywords: | SSR |
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