超滤膜分离白术多糖及其抗氧化活性的研究

利用超滤膜分离装置对白术多糖进行超滤分离,按分子量分成5个级别,用苯酚-硫酸比色法分别测定不同分子量范围多糖的糖含量,结果表明:它们占总糖的比例分别为:≥100 000的占3.39%,30 000～100 000的占10.47%,10000～30 000的占5.42%,6 000～10 000的占20.04%,≤6 000的占60.68%.另外各级多糖均具有抗氧化活性,其中10 000～30000和≤6000分子量的白术多糖对超氧阴离子自由基(O-2·)和羟基自由基(·OH)具有极强的消除作用.

Abstract：

Atractylodes macrocephala polysaccharides were ultrafiltered by an ultrafiltration membrane separation unit, and they were divided into 5 levels in accordance with molecular weight (MW). The contents of polysaccharides for different MW were determined with the phenol-sulfuric acid method. The result showed that the proportion of the polysaccharides with different MW in the total polysaccharides were as follows: 3.39% for MW above 100 000, 10.47% for MW between 30 000 and 100 000, 5.42% for MW between 10 000 and 30 000, 20.04% for MW between 6 000 and 10 000, and 60.68% for MW less than 6000. Determination of antioxidant activity demonstrated that polysaccharides of different MW all had antioxidant activity, and polysaccharides with an MW between 10 000 and 30 000 and less than 6 000, in particular, had a very strong scavenging action on O-2 · and · OH.

Isolation of Atractylodes macrocephala Koidz Polysaccharides with Ultrafiltration Membrane and Study of Their Antioxidant Activity