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Mapping and analysis of QTLs related to seed length and seed width in Glycine max
Authors:Huihui Gao  Yue Gu  Hongwei Jiang  Yingying Li  Dawei Xin  Chunyan Liu  Rongsheng Zhu  Zhaoming Qi  Yong Zhang  Candong Li  Jinxing Wang  Zhenbang Hu  Xiaoxia Wu  Qingshan Chen
Abstract:Both seed length and seed width are important traits for soybean yield. In the present study, 89 Quantitative trait loci (QTLs) of seed length and 65 QTLs of seed width were collected from published papers and our study. QTLs in this study were evaluated by the soymap2, then totally 23 consensus QTLs were located on 17 linkage groups (LGs) through the meta‐analysis. The minimum confidence interval was 0.28 cM and the mean phenotypic variance (R2) was ranged from 5.33% to 23.36%. To optimize these QTLs based on statistic analysis, overview method was further used to narrow down CI, the number of QTLs was narrowed down to 84. Furthermore, 2,750 candidate genes were screened from the consensus QTL intervals by informatics, a total of 37 genes were found to be associated with seed size. All results could lay a foundation for MAS (Molecular Assisted Selection) and gene cloning.
Keywords:candidate genes  consensus QTLs  meta and overview analysis  seed length  seed width  soybean
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