| 橄榄SRAP-PCR体系的建立和优化 |
| |
| 引用本文： | 张平湖 刘冠明. 橄榄SRAP-PCR体系的建立和优化[J]. 中国农学通报, 2010, 26(15): 86-88 |
| |
| 作者姓名： | 张平湖 刘冠明 |
| |
| 作者单位： | 1. 仲恺农业工程学院白云校区办公室,广州,510225
2. 仲恺农业工程学院农学院,广州,510225 |
| |
| 基金项目： | 广东省农业科技计划项目 |
| |
| 摘 要： | 以橄榄品种为材料，采用L16（45）的正交试验设计，对影响PCR反应的Taq酶量、Mg2+浓度、模板DNA含量、dNTPs浓度和引物浓度5个因素进行了SRAP-PCR扩增反应条件优化研究，并利用反应体系对11个橄榄品种进行了SRAP-PCR扩增。结果表明：在20μl体系中，Taq酶1.5U、Mg2+2.5 mmol/L、模板DNA 60ng、dNTPs 0.2 mmol/L和引物0.15μmol/L时的扩增效果最好；利用该体系，SRAP标记引物对Me5- Em2在11个橄榄品种中可以扩增出7条清晰的多态性条带。
|
| 关 键 词： | 加拿大 加拿大 禽流感 补偿政策 启示 |
| 收稿时间： | 2010-02-20 |
| 修稿时间： | 2010-03-15 |
Establishment and Optimization of SRAP-PCR System in Canarium album Reausch |
| |
| Zhang Pinghu,Liu Guanming. Establishment and Optimization of SRAP-PCR System in Canarium album Reausch[J]. Chinese Agricultural Science Bulletin, 2010, 26(15): 86-88 |
| |
| Authors: | Zhang Pinghu Liu Guanming |
| |
| Affiliation: | 1 New Campus Office, Zhongkai Agrotechnical University , Guangzhou 510225; 2 Agronomy College, Zhongkai Agrotechnical University , Guangzhou 510225) |
| |
| Abstract: | The variety of Canarium album Reausch was used as material, optimization of SRAP-PCR system was studied with L 16 （ 4 5 ） orthogonal design of five factors （Taq polymerase, Mg 2 ＋ , DNA template, dNTPs and primer） and the DNA of 11 varieties were amplified with the optimized SRAP-PCR system. The results showed that in 20 μ L amplification system contained 1.5 U Taq DNA polymerase, 2.5 mmol/L Mg 2 ＋ , 60 ng genomic templates DNA, 0.2 mol/L dNTPs, and 0.15 μ mol/L Primer, the result of SRAP-PCR was best for Canarium album Reausch. Seven polymorphic bands were amplified with one primer pair Me5-Em2 through the optimized SRAP-PCR system in 11 varieties of Canarium album Reausch. |
| |
| Keywords: | SRAP PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
|
点击此处可从《中国农学通报》下载全文 |
|
